Name: Anti-Axin 2 antibody [EPR2005(2)]
Brand: RabMAb
SKU: ab109307
Rating: 4 (5 reviews)

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Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2005(2)] to Axin 2
Suitable for: ICC/IF, IHC-P, WB
Reacts with: Mouse, Rat, Human

Carrier Free

製品名

Anti-Axin 2 antibody [EPR2005(2)]
Axin 2 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR2005(2)] to Axin 2
由来種

Rabbit
アプリケーション

適用あり: ICC/IF, IHC-P, WBmore details
種交差性

交差種: Mouse, Rat, Human
交差が予測される動物種: Pig
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: C6, PC-12, NIH/3T3, MCF7, SW480, and PC3 cell lysates. IHC-P: Human lung carcinoma tissue. ICC/IF: LnCap cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR2005(2)
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-Axin 2 antibody [EPR2005(2)] - BSA and Azide free (ab192230)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- SW480 whole cell lysate (ab3957)
- NIH/3T3 whole cell lysate (ab7179)
Recombinant Protein
- Recombinant Human Axin 2 protein (ab204097)
Related Products
- Wnt Pathway Antibody Sampler Panel (ab242226)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab109307の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
ICC/IF	(2)	1/150.
IHC-P	(2)	1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB	(1)	1/1000 - 1/2000. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).

特記事項
ICC/IF 1/150.
IHC-P 1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/2000. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).

機能

Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.
組織特異性

Expressed in brain and lymphoblast.
関連疾患

Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types.
配列類似性

Contains 1 DIX domain.
Contains 1 RGS domain.
ドメイン

The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
翻訳後修飾

Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
細胞内局在

Cytoplasm.
Target information above from: UniProt accession Q9Y2T1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 8313 Human
- Entrez Gene: 12006 Mouse
- Entrez Gene: 29134 Rat
- Omim: 604025 Human
- SwissProt: Q9Y2T1 Human
- SwissProt: O88566 Mouse
- SwissProt: O70240 Rat
- Unigene: 156527 Human
- Unigene: 71710 Mouse
- Unigene: 162212 Rat
see all
別名
- Axil antibody
- Axin like protein antibody
- Axin-2 antibody
- Axin-like protein antibody
- Axin2 antibody
- AXIN2_HUMAN antibody
- Axis inhibition protein 2 antibody
- Conductin antibody
- DKFZp781B0869 antibody
- MGC10366 antibody
- MGC126582 antibody
see all

Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (unpurified)

Lane 1 : SW480 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 94 kDa
Observed band size: 94 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Axin 2 with unpurified ab109307 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)This image is courtesy of an anonymous Abreview

Unpurified ab109307 staining Axin 2 in 293T cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 3% BSA) for 16 hours. An Alexa Fluor^® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
See Abreview
Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (purified)

Lane 1 : SW480 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 94 kDa
Observed band size: 94 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Axin 2 with purified ab109307 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (unpurified)

Lane 1 : MCF7 cell lysates
Lane 2 : SW480 cell lysates
Lane 3 : PC3 cell lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 94 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

Immunocytochemistry/Immunofluorescence analysis of LnCap cells labelling Axin 2 with unpurified ab109307 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/150) and secondary antibody, ab150113, an Alexa Fluor^® 488-conjugated goat anti-mouse IgG (1/500).
Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

Immunocytochemistry/Immunofluorescence analysis of LnCap cells labelling Axin 2 with purified ab109307 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/150) and secondary antibody, ab150113, an Alexa Fluor^® 488-conjugated goat anti-mouse IgG (1/500).
Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

Download

ab109307 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109307 は 34 報の論文で使用されています。

Li J et al. AXIN1 knockout does not alter AMPK/mTORC1 regulation and glucose metabolism in mouse skeletal muscle. J Physiol 599:3081-3100 (2021). PubMed: 33913171
Wang X et al. CORO6 Promotes Cell Growth and Invasion of Clear Cell Renal Cell Carcinoma via Activation of WNT Signaling. Front Cell Dev Biol 9:647301 (2021). PubMed: 34026752
Xie B et al. MicroRNA-1246 by Targeting AXIN2 and GSK-3ß Overcomes Drug Resistance and Induces Apoptosis in Chemo-resistant Leukemia Cells. J Cancer 12:4196-4208 (2021). PubMed: 34093820
Gambini A et al. Developmentally Programmed Tankyrase Activity Upregulates ß-Catenin and Licenses Progression of Embryonic Genome Activation. Dev Cell 53:545-560.e7 (2020). PubMed: 32442396
Meyers CA et al. A Neurotrophic Mechanism Directs Sensory Nerve Transit in Cranial Bone. Cell Rep 31:107696 (2020). PubMed: 32460020

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-5 of 5 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (MDA-MB-231 breast cancer xenograft)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9

Permeabilization

Specification

MDA-MB-231 breast cancer xenograft

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 14 2015

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Sample

Human Cell (293T)

Specification

293T

Permeabilization

Yes - Triton 1%

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 20 2014

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

(agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate pH6

Sample

Mouse Tissue sections (MOUSE EMBRYO 20DPC)

Specification

MOUSE EMBRYO 20DPC

Permeabilization

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 09 2013

Application

Western blot

Sample

Pig Cell lysate - whole cell (PAE cell line (Pig Aortic Endothelial))

Loading amount

70 µg

Specification

PAE cell line (Pig Aortic Endothelial)

Gel Running Conditions

Reduced Denaturing (10)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Raul Peña

Verified customer

投稿 Dec 17 2012

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Embryonic Stem Cells)

Specification

Embryonic Stem Cells

Fixative

Paraformaldehyde

Permeabilization

Yes - PBS 0,3% triton

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mrs. Elisa Pedone

Verified customer

投稿 Nov 27 2012

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

関連疾患

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (34)

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Axin 2 antibody [EPR2005(2)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Axin 2 antibody [EPR2005(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Axin 2 antibody [EPR2005(2)]

Western blot abreview for Anti-Axin 2 antibody [EPR2005(2)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Axin 2 antibody [EPR2005(2)]