Anti-Axin 2 抗体 (ab107613)
Key features and details
- Rabbit polyclonal to Axin 2
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Axin 2 antibody
Axin 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Axin 2 -
由来種
Rabbit -
特異性
ab107613 antibody is predicted to not cross-react with Axin 1. At least four isoforms of Axin 2 are known to exist; this antibody will recognize all four. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Human Axin 2 (C terminal). The immunogen is located within amino acids 780 - 830 of Axin 2.
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ポジティブ･コントロール
- WB: Mouse lung tissue lysate. IHC-P: Mouse lung and colon tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab107613の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 94 kDa.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 94 kDa. |
ターゲット情報
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機能
Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B. -
組織特異性
Expressed in brain and lymphoblast. -
関連疾患
Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types. -
配列類似性
Contains 1 DIX domain.
Contains 1 RGS domain. -
ドメイン
The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2. -
翻訳後修飾
Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 12006 Mouse
- SwissProt: O88566 Mouse
- Unigene: 71710 Mouse
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別名
- Axil antibody
- Axin like protein antibody
- Axin-2 antibody
see all
画像
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Western blot - Anti-Axin 2 antibody (ab107613)Anti-Axin 2 antibody (ab107613) at 1 µg/ml + Mouse lung tissue lysate at 15 µg
Predicted band size: 94 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab107613)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse lung tissue staining Axin 2 with ab107613 at 20 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab107613)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse lung tissue labeling Axin 2 with Anti-Axin 2 antibody (ab107613) at 5μg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab107613)This image is courtesy of an anonymous Abreviewab107613 staining Axin 2 in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500) for 18 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/800) was used as the secondary antibody.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab107613 は 2 報の論文で使用されています。
- Rauner M et al. Transferrin receptor 2 controls bone mass and pathological bone formation via BMP and Wnt signaling. Nat Metab 1:111-124 (2019). PubMed: 30886999
- Ouyang J et al. The role of aplysia ras homolog I in colon cancer cell invasion and adhesion. Exp Ther Med 14:5193-5199 (2017). PubMed: 29201236
