Anti-Avian Influenza Nucleoprotein 抗体 (ab22285)
Key features and details
- Rabbit polyclonal to Avian Influenza Nucleoprotein
- Suitable for: ELISA, ICC/IF
- Reacts with: Dog
- Isotype: IgG
製品の概要
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製品名
Anti-Avian Influenza Nucleoprotein antibody
Avian Influenza Nucleoprotein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Avian Influenza Nucleoprotein -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, ICC/IFmore details -
種交差性
交差種: Dog, Other species -
免疫原
Synthetic peptide, corresponding to amino acids 428-441 of Avian Influenza Nucleoprotein
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22285の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use a concentration of 0.1 µg/ml.
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| ICC/IF |
1/10.
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| 特記事項 |
|---|
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ELISA
Use a concentration of 0.1 µg/ml. |
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ICC/IF
1/10. |
ターゲット情報
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関連性
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus -
細胞内局在
Nuclear -
別名
- NP antibody
- Nucleocapsid protein antibody
- Nucleoprotein antibody
- Protein N antibody
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Avian Influenza Nucleoprotein antibody (ab22285)
Immunofluoroscence staining of influenza−infected MDCK cells using ab22285 at 1:10 dilution.
Image Courtesy of Catherine Thompson, The University of Reading
データシートおよび資料
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Datasheet download
参考文献 (4)
ab22285 は 4 報の論文で使用されています。
- Sharma A et al. Influenza A Virus Nucleoprotein Activates the JNK Stress-Signaling Pathway for Viral Replication by Sequestering Host Filamin A Protein. Front Microbiol 11:581867 (2020). PubMed: 33101257
- Tripathi S et al. Influenza A virus nucleoprotein induces apoptosis in human airway epithelial cells: implications of a novel interaction between nucleoprotein and host protein Clusterin. Cell Death Dis 4:e562 (2013). WB . PubMed: 23538443
- Martín MC et al. Neisseria gonorrhoeae meningitis in pregnant adolescent. Emerg Infect Dis 14:1672-4 (2008). PubMed: 18826848
- Yamamoto Y et al. Detecting avian influenza virus (H5N1) in domestic duck feathers. Emerg Infect Dis 14:1671-2 (2008). PubMed: 18826847
