Anti-Avian Influenza A Neuraminidase 抗体 (ab21304)

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Thank you for your enquiry and your interest.

Tested applications are listed on the on-line product datasheets. Since IHC is not listed on neither of the documents (ab21304 and ab110599), this means that these two productsare not tested and characterized in this specific application. However, this does not necessary imply that the products are unsuitable but we do not have any supporting data yet.

Since both antibodies work in ELISA - an application which recognize the epitope(s) in the natured confirmation - it is very likely that they detect the targets in IHC as well.

If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for kindly providing the sequence. This has been checked, and has the following percentage alignment with the following two antibodies: ab21304 Avian Influenza A Neuraminidase antibody: 80% ab21305 Avian Influenza A Neuraminidase antibody: 87% We recommend alignment shoudl be over 85% to predict that an antibody will detect. Therefore, there is likely to be some crossreactivity with ab21304, but ab21305 may be a better choice based on the alignment scores. Please check the online datasheet to ensure this has been tested in the application the customer would like to use. Click here (or use the following: https://www.abcam.com/Avian-Influenza-A-Neuraminidase-antibody-ab21305.html). I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

Thank you for your enquiry. Unfortunately, the immunogen sequences for ab21292, ab21297, ab21304 and ab21305 are proprietary. However, I can give you a range from which we selected the peptides. ab21305 - residues 150-200 ab21304 – residues 400-450 ab21292 – residues 300-350 ab21297 – residues 100-150 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. A BLAST search on the sequence reveals that Ab21304 should cross-react with neuraminidase from the following strains: H1N1,H4N1, H6N1, H7N1, H9N1 and H11N1. If you have any further questions then please get back in contact with me.

Thank you for getting back to me. Once again, unfortunately I cannot comment on the peptide sequence that this antiserum was raised against as it remains proprietary. However, given the sequence alignment that you have performed it seems that there is significant sequence similarity, in particular within the C terminal end (residues 400-469 show only two differences, both highly conserved). Given this I would expect this antiserum to work against the 1918 Spanish flu. Furthermore given that this is a polyclonal it is even more likely to accommodate any subtle change in epitope should the epitope lie within one of the two residue differences. I hope this information helps. Please do not hesitate to contact me should you require further assistance.

Thank you for your enquiry. Unfortunately the immunogen for this antibody remains proprietary. The peptide sequence used to generate ab21304 is not from the very C-terminus, but from the C-terminal region. It has 13/15 (*******:*****.*) identical residues, 1 highly conserved and 1 conserved when compared to the corresponding region in human InFluenza Virus NA. I was unable to find the sequence for 1918 Spanish Flu NA in NCBI's database. However, I did some searches for it through Google and found a putative sequence through literature (AF117241). If this is the sequence for the 1918 Spanish Flu NA protein there are only two identical residues, and 6 conserved residues: * : :*::: : Please note that AF117241 has very low similarity with the CAC95053 sequence: http://www.ebi.ac.uk/cgi-bin/clustalw/result?tool=clustalw&jobid=clustalw-20051004-18542419&poll=yes I hope this information helps. Please do not hesitate to contact me should you require further assistance.