Anti-Avian Influenza A Hemagglutinin 抗体 (ab21297)

I can confirm that ab21292 only recognizes the full-length H5 and ab21297 will recognize a cleaved subunit. Unfortunately, I cannot disclose the exact peptide sequence. I hope this information will help you,

Thank you for your message and your valuable feedback which has now been passed on to me in the Technical Support department. I am very sorry to hear you have had problems obtaining satisfactory results using these antibodies. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 90 days of purchase), and if it appears that the antibody is at fault, a credit note/refund will be offered. If you would like some technical support I enclose a link to our questionnaires which will help you put your protocol information together very easily so we can look into the details of the experiment and provide some help. Although ab21297 and ab21292 are used in ELISA, ab2285 can be used in several applications. I have therefore provided links to each type of application so you can choose the correct one to fill out for each antibody. https://www.abcam.com/index.html?section=elisa&pageconfig=technical&mode=questionaire https://www.abcam.com/index.html?section=western&pageconfig=technical&mode=questionaire https://www.abcam.com/index.html?section=ihc&pageconfig=technical&mode=questionaire https://www.abcam.com/index.html?section=ip&pageconfig=technical&mode=questionaire Thank you for your time. I look forward to receiving the completed questionnaires.

Thank you for your enquiry. I have been in touch with the source of this antibody and I can tell you that unfortunately the immunogen sequence is proprietary. However, I can tell you that in their opinion the antibody should not cross-react to other Hemagglutinins. Please let me know if you have any more questions.

Thank you for your enquiry. Unfortunately, the originator of the antibody will not disclose this information. The most specific information I can give you is what is listed on our datasheet, that the immunogen was selected from between residues 100-150 of Genbank accession number AAT76166. I am sorry I could not be more helpful. Please do not hesitate to contact us again if you need anything further.

Thank you for your enquiry. I have performed an alignment of the immunogen sequence with accession numbers AAW80717, AAT39066, and AAA43083. I can tell you that the immunogen was selected from a conserved region of the sequence and shows the following sequence similarity: AAW80717: *************** (100% homology) AAT39066: *************** (100% homology) AAA43083: *************** (100% homology) Please let me know if you have any more questions.

Thank you for your enquiry. Unfortunately I cannot provide you with details of the accession number that was used for the design of the immunogen for this antibody. However, I can perform an alignment of your H5 amino acid sequence with the immunogen sequence and provide you with details of the degree of sequence similarity. If you would like me to do this please e-mail me the accession number of your H5 sequence. I look forward to hearing from you.

Thank you for your enquiry. Unfortunately, the immunogen sequences for ab21292, ab21297, ab21304 and ab21305 are proprietary. However, I can give you a range from which we selected the peptides. ab21305 - residues 150-200 ab21304 – residues 400-450 ab21292 – residues 300-350 ab21297 – residues 100-150 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. I can not provide you with the exact sequence used, but it is 14 amino acids within residues 100-150 for ab21297 and within residues 300-350 for catalog number ab21292. Please let me know if you have any more questions.

Thank you for your enquiry. Unfortunately I do not have details of any cross-reactivity by this antiserum against non-H5N1 hemagglutinin proteins. I have blasted the peptide sequence of the 15 N-terminal residues: MERIVIALAIISIVK derived from the EXPASY database: http://us.expasy.org/uniprot/Q7TEA9 and there are no results suggesting that this sera was raised using a specific immunogen. I am sorry I cannot be of more help.

Thank you for your enquiry. Ab21297 was generated using a synthetic peptide corresponding to 15 amino acids at the N-terminus of the Hemagglutinin protein. The exact sequence is proprietary. The antibody's specifity was tested by peptide ELISA in which the antibody was able detect 10 ng of free peptide at 1 µg/ml. The antibody reacts with Avian Influenza A (H5N1) Hemagglutinin. If you have any additional questions, please contact us again.