Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (NHDF)
Permeabilization
Yes - 0.5% Triton
Specification
NHDF
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.03% Triton
Dilution
1/50
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
This antibody worked fine for IF. It is not as robust nor as clean as the rabbit AURKB antibody, but is the best mouse AURKB antibody that I have tried.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dana Mitchell
Verified customer
投稿 Nov 02 2020