Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A431 (epithelial))
Specification
A431 (epithelial)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1%
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 1% BSA made up in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 647 Donkey anti Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/500
Additional data
Additional Notes
All antibody incubations and blocking was carried out at room temperature. Very good staining of A431s at cytokinesis. However the staining achieved at metaphase was rather diffuse and unclear, perhaps PFA fixation is not the best way to achieve good staining at this stage.
Image submitted (Tubulin in green, Aurora B in pink, DNA in blue)
Image submitted (Tubulin in green, Aurora B in pink, DNA in blue)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Lux Fatimathas
Verified customer
投稿 Mar 04 2008