Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human coronary artery endothelial cells HCAEC)
Permeabilization
Yes - 0.1% Trition X-100 in HBSS
Specification
Human coronary artery endothelial cells HCAEC
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 2% FBS in HBSS
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Alexa Fluor 488 donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 19 2020