Anti-AU1 tag 抗体 (ab3401)

Submit a review Q&A (4)References (11)

Key features and details

  • Rabbit polyclonal to AU1 tag
  • Suitable for: ELISA, ICC, WB, IP
  • Reacts with: Species independent
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-AU1 tag antibody

  • 製品の詳細

    Rabbit polyclonal to AU1 tag

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ELISA, ICC, WB, IPmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Full length native AU1 (purified):

    DTYRYI

    conjugated to KLH.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.20
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/100 - 1/150. Primary: 1/1000 - 1/30000.
ICC 1/200 - 1/2000.
WB 1/1000 - 1/30000.
IP Use at an assay dependent dilution.

ターゲット情報

  • 別名

    • AU1 epitope tag antibody
    • DTYRYI epitope tag antibody
    • DTYRYI tag antibody

画像

  • Western blot - Anti-AU1 tag antibody (ab3401)
    Western blot - Anti-AU1 tag antibody (ab3401)

    The AU1 tag antibody (ab3401) was used in a Western blot to detect expression of mutant mammalian target of rapamycin (mTOR) proteins.  U373 and A172 human glioma cell lines were transfected with constructs encoding either an AU1 tagged rapamycin resistant (RR) mTOR or AU1 tagged kinase dead (KD) mTOR.  This detected the expression of a 290 kD protein corresponding to the AU1 tagged mutant mTOR proteins in both cell lines.  (The mutant mTOR proteins appear beneath a band corresponding to the wild-type (WT) mTOR protein - this was not detected by ab3401 but a different antibody).

    Picture from review by Amith Panner, submitted 29 July 2004.

    The AU1 tag antibody (ab3401) was used in a Western blot to detect expression of mutant mammalian target of rapamycin (mTOR) proteins. U373 and A172 human glioma cell lines were transfected with constructs encoding either an AU1 tagged rapamycin resistant (RR) mTOR or AU1 tagged kinase dead (KD) mTOR. This detected

参考文献 (11)

ab3401 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3401 は 11 報の論文で使用されています。

  • Raina A  et al. Neuronal Trans-differentiation by Transcription Factors Ascl1 and Nurr1: Induction of a Dopaminergic Neurotransmitter Phenotype in Cortical GABAergic Neurons. Mol Neurobiol N/A:N/A (2019). PubMed: 31317490
  • Ivicak-Kocjan K  et al. Extension and refinement of the recognition motif for Toll-like receptor 5 activation by flagellin. J Leukoc Biol 104:767-776 (2018). PubMed: 29920759
  • Chen G  et al. Bri2 BRICHOS client specificity and chaperone activity are governed by assembly state. Nat Commun 8:2081 (2017). PubMed: 29234026
  • Forstneric V  et al. The role of the C-terminal D0 domain of flagellin in activation of Toll like receptor 5. PLoS Pathog 13:e1006574 (2017). PubMed: 28827825
  • Kuslansky Y  et al. Ubiquitin ligase E6AP mediates nonproteolytic polyubiquitylation of ß-catenin independent of the E6 oncoprotein. J Gen Virol 97:3313-3330 (2016). PubMed: 27902311
View all Publications for this product

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Question

Dear Technical Team,

Hope this e-mail finds you well!

Kindly advise if the item Anti-AU1 tag antibody (ab3401) can be used in Confocal microscopy as enquired by one of our customer.

Looking forward towards your valued response on it.

With Best Regards,

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Answer

Thank you for your enquiry and interest in our products.

This antibody has been tested in ICC so it is very likely to work in Confocal microscopy, though it has not been specifically characterized in this application.

If you need any further assistance in the future, please do not hesitate to contact me.

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Question

we bought from your companyhttps://www.abcam.com/index.html?datasheet=3401but we get no signal in Western Blot: antibodies were used at the recommended dilution in TBST+Milk

My student put a the epitope tag at the N-termini of their construct but instead of putting MDTYRYI they putMADTYRYI.

Is there a possibility that the Alanine prevents the recognition of the epitope by the antibodies knowing that normally the epitope recognized isDTYRYI?

Does the anti-AU1 works better inTBST+BSA?

Thank you for helping us out

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Answer

Je vous contacte suite aux questions auxquelles vous avez répondues il y a quelques jours concernant la qualité des produits et des services Abcam. Je vous remercie d'avoir pris le temps de répondre à ces questions.

J'ai compris que, malgré nos recommendations,vous rencontrez toujours des problèmes avec l'anticorps anti-AU1 tag référenceab3401.

Je serai ravi derésoudre ce problème en vous remboursant, vous faire un avoir, ou vous envoyer un anticorps de remplacement.

En échange, nous serions ravis si vous pouviez nous communiquer quelques détails concernant votre protocole en remplissant le questionnaire technique ci-joint, ces informations sont très importantes pour nous car elles nous permettent d'investiguer la source du problème rencontré avec cet anticorps et de prendre les dispositions nécessaires pour assurer une bonne qualité de nos produits. Merci d'indiquer également le numéro du lot que vous avez reçu, ceci me permettra de déterminer si un autre lot de ab3401 est actuellement disponible.

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Question

HI   we bought from your company Anti-AU1 tag antibody (ab3401) but we get no signal in Western Blot: antibodies were used at the recommended dilution in TBST+Milk   My student put a the epitope tag at the N-termini of their construct but instead of putting MDTYRYI they put MADTYRYI.   Is there a possibility that the Alanine prevents the recognition of the epitope by the antibodies knowing that normally the epitope recognized is DTYRYI ? Does the anti-AU1 works better in  TBST+BSA?   Thank you for helping us out    

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Answer

Merci de nous avoir contactés. Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab3401. L'immunogène ayant servi à produire cet anticorps est DTYRYI. Je ne pense pas que la présence de 2 résidus avant cette séquence vienne perturber la reconnaissance du tag. Vous pouvez en effet essayer d'utiliser comme solution bloquante de la BSA. Une incubation sur la nuit à 4°C est peut-être nécessaire, c'est peut-être quelque chose que vous avez déjà tenté. Si vous n'obtenez toujours pas de détection dans ces conditions et que ce produit a été commandé dans les derniers 6 mois, nous nous ferons un plaisir de vous offrir un remplacement gratuit (avec une référence alternative, ab3400) ou un avoir en compensation. Pourriez-vous, s'il vous plait, m'envoyer le numéro de lot ainsi que le numéro de votre commande? J'espère que ces informations vous aideront. Si nos suggestions ne permettent pas d'améliorer vos résultats, n'hésitez pas à me recontacter pour plus d'assistance.

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Question

Thanks for your response. What exactly are the AU1-AU5 tags (ab3401 etc)? These appear to me to be synthetic sequence against which antibodies have been raised and would (if I'm getting this right) be perfect for my needs.

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Answer

The AU1-AU5 tags are epitope tags and are fairly uncommon. In the instance of ab3401, the sequence DTYRYI is the sequence of the tag. If you have any more questions, please contact us again.

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