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Anti-ATXN2L 抗体 (ab99304)

Submit a review Q&A (1)
Western blot - Anti-ATXN2L antibody (ab99304)
  • Immunoprecipitation - Anti-ATXN2L antibody (ab99304)

Key features and details

  • Rabbit polyclonal to ATXN2L
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-ATXN2L antibody
    ATXN2L 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to ATXN2L

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant

  • 免疫原

    Synthetic peptide within Human ATXN2L aa 150-200. The exact sequence is proprietary.
    Database link: NP_009176.2

  • ポジティブ･コントロール

    • WB: HeLa and 293T whole cell lysate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab99304の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP
Use at 2-5 µg/mg of lysate.
特記事項
WB
1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP
Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 組織特異性

    Expressed at high levels in thymus, lymph node, spleen, fetal kidney and adult testis. Constitutively associated with MPL and EPOR in hematopoietic cells.

  • 配列類似性

    Belongs to the ataxin-2 family.

  • 翻訳後修飾

    Thrombopoietin triggers the phosphorylation on tyrosine residues in a way that is dependent on MPL C-terminal domain.
    Arg-361 is dimethylated, probably to asymmetric dimethylarginine.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • A2D antibody
      • A2LG antibody
      • A2LP antibody
      • A2RP antibody
      • Ataxin 2 domain protein antibody
      • Ataxin 2 like antibody
      • Ataxin 2 like protein antibody
      • Ataxin 2 related protein antibody
      • Ataxin-2 domain protein antibody
      • Ataxin-2 like antibody
      • Ataxin-2-like protein antibody
      • Ataxin-2-related protein antibody
      • ATX2L_HUMAN antibody
      • ATXN2L antibody
      • ATXN2L ataxin 2 like antibody
      see all

    画像

    • Western blot - Anti-ATXN2L antibody (ab99304)
      Western blot - Anti-ATXN2L antibody (ab99304)
      All lanes : Anti-ATXN2L antibody (ab99304) at 0.04 µg/ml

      Lane 1 : Hela whole cell lysate at 50 µg
      Lane 2 : Hela whole cell lysate at 15 µg
      Lane 3 : Hela whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 114 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-ATXN2L antibody (ab99304)
      Immunoprecipitation - Anti-ATXN2L antibody (ab99304)

      ab99304, at 0.1 µg/ml, staining ATXN2L in HeLa whole cell lysate immunoprecipitated using ab99304 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.

    参考文献 (0)

    ab99304 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab99304 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Is this antibody predicted to react with mouse Atxn2L?

    Read More
    Answer

    Thank you very much for your interest our AbTrial program.

    While ab99304 has not been tested for use on mouse samples, the immunogen is 100% homologous with the mouse sequence. With our AbTrial program, you can now use our products in an untested application or species without financial risk.

    Simply follow these easy steps below to apply for our AbTrial Program:

    1. Reply to this email, letting us know you are interested in testing this product.

    2. Our scientists will email you an inactive personal discount code for the value of the product.

    3. Purchase and test the product at the regular price.

    4. Submit your results, including your discount code in the additional notes section of your Abreview.

    5. Once the Abreview is submitted, the discount code will become active.

    6. Apply your discount code on your next order to receive that value off.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com