Anti-ATXN2L 抗体 (ab99304)
Key features and details
- Rabbit polyclonal to ATXN2L
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ATXN2L antibody
ATXN2L 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ATXN2L -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide within Human ATXN2L aa 150-200. The exact sequence is proprietary.
Database link: NP_009176.2 -
ポジティブ・コントロール
- WB: HeLa and 293T whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99304の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 114 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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組織特異性
Expressed at high levels in thymus, lymph node, spleen, fetal kidney and adult testis. Constitutively associated with MPL and EPOR in hematopoietic cells. -
配列類似性
Belongs to the ataxin-2 family. -
翻訳後修飾
Thrombopoietin triggers the phosphorylation on tyrosine residues in a way that is dependent on MPL C-terminal domain.
Arg-361 is dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 11273 Human
- Entrez Gene: 233871 Mouse
- Entrez Gene: 361649 Rat
- Omim: 607931 Human
- SwissProt: Q8WWM7 Human
- SwissProt: Q7TQH0 Mouse
- Unigene: 460499 Human
- Unigene: 231450 Mouse
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別名
- A2D antibody
- A2LG antibody
- A2LP antibody
see all
画像
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All lanes : Anti-ATXN2L antibody (ab99304) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 114 kDa
Exposure time: 30 seconds -
ab99304, at 0.1 µg/ml, staining ATXN2L in HeLa whole cell lysate immunoprecipitated using ab99304 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab99304 は論文での使用が確認できていません。