製品の概要

  • 製品名
    Anti-ATXN2L antibody
    ATXN2L 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ATXN2L
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide within Human ATXN2L aa 150-200. The exact sequence is proprietary.
    Database link: NP_009176.2

  • ポジティブ･コントロール
    • WB: HeLa and 293T whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG

アプリケーション

Our Abpromise guarantee covers the use of ab99304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 組織特異性
    Expressed at high levels in thymus, lymph node, spleen, fetal kidney and adult testis. Constitutively associated with MPL and EPOR in hematopoietic cells.
  • 配列類似性
    Belongs to the ataxin-2 family.
  • 翻訳後修飾
    Thrombopoietin triggers the phosphorylation on tyrosine residues in a way that is dependent on MPL C-terminal domain.
    Arg-361 is dimethylated, probably to asymmetric dimethylarginine.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • A2D antibody
    • A2LG antibody
    • A2LP antibody
    • A2RP antibody
    • Ataxin 2 domain protein antibody
    • Ataxin 2 like antibody
    • Ataxin 2 like protein antibody
    • Ataxin 2 related protein antibody
    • Ataxin-2 domain protein antibody
    • Ataxin-2 like antibody
    • Ataxin-2-like protein antibody
    • Ataxin-2-related protein antibody
    • ATX2L_HUMAN antibody
    • ATXN2L antibody
    • ATXN2L ataxin 2 like antibody
    see all

画像

  • Western blot - Anti-ATXN2L antibody (ab99304)
    Western blot - Anti-ATXN2L antibody (ab99304)
    All lanes : Anti-ATXN2L antibody (ab99304) at 0.04 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 114 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation - Anti-ATXN2L antibody (ab99304)
    Immunoprecipitation - Anti-ATXN2L antibody (ab99304)

    ab99304, at 0.1 µg/ml, staining ATXN2L in HeLa whole cell lysate immunoprecipitated using ab99304 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.

参考文献

ab99304 has not yet been referenced specifically in any publications.

ab99304 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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Is this antibody predicted to react with mouse Atxn2L?

Thank you very much for your interest our AbTrial program.

While ab99304 has not been tested for use on mouse samples, the immunogen is 100% homologous with the mouse sequence. With our AbTrial program, you can now use our products in an unte...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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