Anti-ATR 抗体 (ab2905)
Key features and details
- Rabbit polyclonal to ATR
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ATR antibody
ATR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ATR -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Fusion protein, corresponding to amino acids 400-460 of Human ATR.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 305 kDa (predicted molecular weight: 309 kDa). (in lysate from UV irradiated K562 cells). |
ターゲット情報
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機能
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. -
組織特異性
Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary. -
関連疾患
Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance. -
配列類似性
Belongs to the PI3/PI4-kinase family. ATM subfamily.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain. -
翻訳後修飾
Phosphorylated; autophosphorylates in vitro. -
細胞内局在
Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling. - Information by UniProt
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参照データベース
- Entrez Gene: 545 Human
- Omim: 601215 Human
- SwissProt: Q13535 Human
- Unigene: 271791 Human
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別名
- Ataxia telangiectasia and Rad3 related antibody
- Ataxia telangiectasia and Rad3-related protein antibody
- ATR antibody
see all
画像
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Western blot - Anti-ATR antibody (ab2905)Western blot on ATR in K562 whole cell lysate using ab2905. -
Western blot - Anti-ATR antibody (ab2905)All lanes : Anti-ATR antibody (ab2905) at 1/1000 dilution
Lane 1 : MCF10A breast epithelial cells, grown in normoxic conditions (20% O2). Cells were lysed with NP40.
Lane 2 : MCF10A Breast Epithelial cells grown in hypoxic. conditions (5% O2). Cells were lysed with NP40.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti Rabbit (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 309 kDa
Observed band size: 300 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Blocking: Carried out using non fat dry milk for 1 hr at room temperature.
This image is an edited version of an image submitted courtesy of an Abreview by Francisco Ramirez-Valle submitted on 15th November 2005. We do not have any further information relating to this image.
データシートおよび資料
参考文献 (29)
ab2905 は 29 報の論文で使用されています。
- Zheng T et al. RBMX is required for activation of ATR on repetitive DNAs to maintain genome stability. Cell Death Differ N/A:N/A (2020). PubMed: 32494026
- Kim W et al. ZFP161 regulates replication fork stability and maintenance of genomic stability by recruiting the ATR/ATRIP complex. Nat Commun 10:5304 (2019). PubMed: 31757956
- Li J & Zheng J Theaflavins prevent cartilage degeneration via AKT/FOXO3 signaling in vitro. Mol Med Rep 19:821-830 (2019). PubMed: 30569095
- Meng X et al. DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma. EBioMedicine 41:185-199 (2019). PubMed: 30773478
- Huang J et al. Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2. Cell Rep 27:1794-1808.e5 (2019). PubMed: 31067464
