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  1. Link

    atpb-antibody-3d5-mitochondrial-marker-ab14730.pdf

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Tags & Cell Markers Subcellular Markers Organelles Mitochondria
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Anti-ATPB 抗体 [3D5] - Mitochondrial Marker (ab14730)

  • Datasheet
  • SDS
Reviews (40)Q&A (6)References (202)

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Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
  • Flow Cytometry - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
  • Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
  • Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

Key features and details

  • Mouse monoclonal [3D5] to ATPB - Mitochondrial Marker
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Cow, Human, Caenorhabditis elegans
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 HRP

こちらの製品もご検討ください

一次抗体
Product image
Anti-SDHA antibody [EPR9043(B)] (ab137040)
一次抗体
Product image
Alexa Fluor® 488 Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197904)
タンパク質
Product image
Recombinant Human ATPB protein (Tagged) (ab235856)

関連製品

製品の概要

  • 製品名

    Anti-ATPB antibody [3D5] - Mitochondrial Marker
    ATPB 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [3D5] to ATPB - Mitochondrial Marker
  • 由来種

    Mouse
  • 特異性

    Human and Bovine complex V beta subunit (ATPB).
  • アプリケーション

    適用あり: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Cow, Human, Caenorhabditis elegans
    交差が予測される動物種: Goat, Cat, Dog, Pig, Common marmoset
  • 免疫原

    Tissue, cells or virus corresponding to Human ATPB.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituent: HEPES buffered saline
  • Concentration information loading...
  • 精製度

    IgG fraction
  • 特記事項(精製)

    Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • ポリ/モノ

    モノクローナル
  • クローン名

    3D5
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    kappa
  • 研究分野

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex V
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Alternative Versions

    • Alexa Fluor® 647 Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197649)
    • Alexa Fluor® 488 Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197904)
    • HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab197905)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human ATPB protein (Tagged) (ab235856)
  • Related Products

    • Mitochondrial Staining Kit - Red Fluorescence - Cytopainter (ab112145)
    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Mitochondrial Staining Kit - NIR Fluorescence - Cytopainter (ab176747)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab14730の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (13)
Use a concentration of 0.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ICC/IF (8)
Use a concentration of 1 - 2 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P (10)
Use at an assay dependent concentration.
特記事項
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ICC/IF
Use a concentration of 1 - 2 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • 配列類似性

    Belongs to the ATPase alpha/beta chains family.
  • 細胞内局在

    Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
  • Target information above from: UniProt accession P06576 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 327675 Cow
    • Entrez Gene: 403669 Dog
    • Entrez Gene: 506 Human
    • Entrez Gene: 11947 Mouse
    • Entrez Gene: 100157156 Pig
    • Entrez Gene: 171374 Rat
    • GenBank: BC016512 Human
    • Omim: 102910 Human
    • SwissProt: P00829 Cow
    • SwissProt: P06576 Human
    • SwissProt: P56480 Mouse
    • SwissProt: P10719 Rat
    • Unigene: 406510 Human
    • Unigene: 238973 Mouse
    • Unigene: 92965 Rat
    see all
  • 別名

    • ATP 5B antibody
    • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
    • ATP synthase subunit beta mitochondrial antibody
    • ATP synthase subunit beta, mitochondrial antibody
    • atp5b antibody
    • ATPB antibody
    • ATPB_HUMAN antibody
    • ATPMB antibody
    • ATPSB antibody
    • Epididymis secretory protein Li 271 antibody
    • HEL-S-271 antibody
    • Mitochondrial ATP synthase beta subunit antibody
    • Mitochondrial ATP Synthase Subunit Beta antibody
    • Mitochondrial ATP synthetase beta subunit antibody
    see all

画像

  • Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    All lanes :

    Lane 1 : HeLa
    Lane 2 : HepG2
    Lane 3 : Hu liver
    Lane 4 : Ms liver

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 52 kDa



    Merged signal (red and green). Green - ab14730 observed at 52 kDa. Red - loading control, ab181602 observed at 37 kDa.

    Samples were subjected to SDS-PAGE. ab14730 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1ug/mL and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    ab14730 staining ATPB in cultured human fibroblasts. Cells were fixed, permeabilized and then labelled with ab14730 followed by an AlexaFluor® 488-conjugated Goat anti-Mouse IgG1-specific secondary antibody (2 µg/ml)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    ab14730 (2µg/ml) staining ATPB in human duodenum using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and mitochondrial staining of epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Flow Cytometry - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    Flow Cytometry - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    ab14730 (blue) at 1µg/ml staining ATPB in HL-60 cells and analyzed by Flow cytometry. Red histogram represents equal quantity of isotype control.
  • Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    All lanes : Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

    Lane 1 : isolated mitochondria from human heart at 5 µg
    Lane 2 : isolated mitochondria from bovine heart at 1 µg
    Lane 3 : isolated mitochondria from rat heart at 10 µg
    Lane 4 : isolated mitochondria from mouse heart at 10 µg

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    All lanes : Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730) at 0.8 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

プロトコール

  • Mouse on Mouse staining protocol

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (202)

ab14730 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab14730 は 202 報の論文で使用されています。

  • Visuttijai K  et al. Progressive external ophthalmoplegia associated with novel MT-TN mutations. Acta Neurol Scand 143:103-108 (2021). PubMed: 32869280
  • Chen C  et al. Imaging mass cytometry reveals generalised deficiency in OXPHOS complexes in Parkinson's disease. NPJ Parkinsons Dis 7:39 (2021). PubMed: 33980828
  • Seale LA  et al. Adaptive Thermogenesis in a Mouse Model Lacking Selenoprotein Biosynthesis in Brown Adipocytes. Int J Mol Sci 22:N/A (2021). PubMed: 33435397
  • Fogo GM  et al. Machine learning-based classification of mitochondrial morphology in primary neurons and brain. Sci Rep 11:5133 (2021). PubMed: 33664336
  • Zhang C & Boppart SA Tracking the formation and degradation of fatty-acid-accumulated mitochondria using label-free chemical imaging. Sci Rep 11:6671 (2021). PubMed: 33758233
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 46 Abreviews or Q&A

Western blot abreview for Anti-ATPB antibody [3D5] - Mitochondrial Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (20ul of 60ul total protein extract from 4 whole fl)
Gel Running Conditions
Reduced Denaturing (4-12% BIS-TRIS PLUS)
Loading amount
1.3 cells
Specification
20ul of 60ul total protein extract from 4 whole fl
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 May 11 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ATPB antibody [3D5] - Mitochondrial Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Kidney)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Sep 18 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ATPB antibody [3D5] - Mitochondrial Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Sep 15 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ATPB antibody [3D5] - Mitochondrial Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Sep 15 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ATPB antibody [3D5] - Mitochondrial Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Common marmoset Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Sep 15 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ATPB antibody [3D5] - Mitochondrial Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Sep 15 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ATPB antibody [3D5] - Mitochondrial Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Sep 15 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ATPB antibody [3D5] - Mitochondrial Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Goat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Sep 15 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ATPB antibody [3D5] - Mitochondrial Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Sep 15 2021

Western blot abreview for Anti-ATPB antibody [3D5] - Mitochondrial Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
10 µg
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

DR. Tom Ryan

Verified customer

投稿 Dec 17 2020

1-10 of 46 Abreviews or Q&A

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