Anti-ATP6V0A1 抗体 (ab105937)
Key features and details
- Rabbit polyclonal to ATP6V0A1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ATP6V0A1 antibody
ATP6V0A1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ATP6V0A1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Cow, Pig
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免疫原
Synthetic peptide corresponding to a region within C terminal amino acids 671-720 (TLNFGGIRVG NGPTEEDAEI IQHDQLSTHS EDAEEPTEDE VFDFGDTMVH) of Mouse ATP6V0A1 (NP_058616).
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ポジティブ･コントロール
- WB: DLD1 cell lysate; Mouse uterus and skeletal muscle lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105937の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 96 kDa.
Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
| 特記事項 |
|---|
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WB
Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 96 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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機能
Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle. -
配列類似性
Belongs to the V-ATPase 116 kDa subunit family. -
細胞内局在
Cytoplasmic vesicle membrane. Melanosome. Coated vesicle. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 286768 Cow
- Entrez Gene: 535 Human
- Entrez Gene: 11975 Mouse
- Entrez Gene: 29757 Rat
- Omim: 192130 Human
- SwissProt: Q29466 Cow
- SwissProt: Q93050 Human
- SwissProt: Q9Z1G4 Mouse
see all -
別名
- a1 antibody
- ATP6N1 antibody
- ATP6N1A antibody
see all
画像
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Western blot - Anti-ATP6V0A1 antibody (ab105937)Anti-ATP6V0A1 antibody (ab105937) (0.2-1 µg/ml) + Mouse Uterus
Predicted band size: 96 kDa -
Western blot - Anti-ATP6V0A1 antibody (ab105937)Anti-ATP6V0A1 antibody (ab105937) at 1 µg/ml + DLD1 cell lysate at 10 µg
Predicted band size: 96 kDaWestern blot analysis of DLD1 cell lysate labeling ATP6V0A1 with ab105937.
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Western blot - Anti-ATP6V0A1 antibody (ab105937)Anti-ATP6V0A1 antibody (ab105937) at 1 µg/ml + Mouse skeletal muscle lysate at 10 µg
Predicted band size: 96 kDaWestern blot analysis of mouse skeletal muscle lysate labeling ATP6V0A1 with ab105937.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab105937 は 4 報の論文で使用されています。
- Chitirala P et al. Role of V-ATPase a3-Subunit in Mouse CTL Function. J Immunol 204:2818-2828 (2020). PubMed: 32269094
- Kelly AC et al. Adrenergic receptor stimulation suppresses oxidative metabolism in isolated rat islets and Min6 cells. Mol Cell Endocrinol 473:136-145 (2018). WB . PubMed: 29360563
- Wang X et al. Defective lysosomal clearance of autophagosomes and its clinical implications in nonalcoholic steatohepatitis. FASEB J 32:37-51 (2018). PubMed: 28842428
- Valapala M et al. Lysosomal-mediated waste clearance in retinal pigment epithelial cells is regulated by CRYBA1/ßA3/A1-crystallin via V-ATPase-MTORC1 signaling. Autophagy 10:480-96 (2014). PubMed: 24468901
