Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse brain)
Permeabilization
Yes - 0.5% TX100
Specification
mouse brain
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% TX100, 1% goat serum, 1X PBS
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: goat anti mouse Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
Epitope unmasking was performed using citrate buffer with pH 6.0 and 15 min heat treatment (90C).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
投稿 Dec 28 2021