Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - 0.5% triton-X100
Specification
U2OS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
0 minute(s)
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Lawrence Welch
Verified customer
投稿 Jun 15 2019