Anti-ATP1B1 抗体 [M17-P5-F11] (ab2873)

Reviews (1)Q&A (2)References (13)

Key features and details

  • Mouse monoclonal [M17-P5-F11] to ATP1B1
  • Suitable for: IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Sheep, Dog, Human, Pig, Drosophila melanogaster
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-ATP1B1 antibody [M17-P5-F11]
    ATP1B1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [M17-P5-F11] to ATP1B1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, ICC/IF, Flow Cytmore details

  • 種交差性

    交差種: Mouse, Rat, Sheep, Dog, Human, Pig, Drosophila melanogaster
    交差が予測される動物種: Rabbit, Guinea pig, Chimpanzee, Cynomolgus monkey

  • 免疫原

    Full length native protein (purified) corresponding to Sheep ATP1B1. Purified lamb kidney sodium/potassium ATPase beta.

  • エピトープ

    This antibody recognizes an epitope between amino acid residues 195-199 of sheep sodium/potassium ATPase beta 1.

  • 特記事項

     This product was previously labelled as beta 1 Sodium Potassium ATPase

     

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200.
ICC/IF 1/100 - 1/1000.
Flow Cyt 1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)
    IHC image of ab2873 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2873, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

    Immunocytochemistry/Immunofluorescence analysis of ATP1B1 shows staining in HeLa cells. ATP1B1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2873 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

    Immunocytochemistry/Immunofluorescence analysis of ATP1B1 shows staining in MCF-7 cells. ATP1B1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2873 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

    Immunocytochemistry/Immunofluorescence analysis of ATP1B1 shows staining in U251 cells. ATP1B1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2873 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

  • Flow Cytometry - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)
    Flow Cytometry - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)
    Overlay histogram showing HEK293 cells stained with ab2873 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2873, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase beta ab2873 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human liver tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase beta ab2873 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase beta ab2873 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

参考文献 (13)

ab2873 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2873 は 13 報の論文で使用されています。

  • Nepal N  et al. Unique Regulation of Na-K-ATPase during Growth and Maturation of Intestinal Epithelial Cells. Cells 8:N/A (2019). PubMed: 31208048
  • Scudieri P  et al. Increased expression of ATP12A proton pump in cystic fibrosis airways. JCI Insight 3:N/A (2018). PubMed: 30333310
  • Schiapparelli P  et al. NKCC1 Regulates Migration Ability of Glioblastoma Cells by Modulation of Actin Dynamics and Interacting with Cofilin. EBioMedicine 21:94-103 (2017). ICC/IF ; Human . PubMed: 28679472
  • Liu W  et al. The Human "Cochlear Battery" - Claudin-11 Barrier and Ion Transport Proteins in the Lateral Wall of the Cochlea. Front Mol Neurosci 10:239 (2017). PubMed: 28848383
  • Wang SH  et al. Expression and potential roles of sodium-potassium ATPase and E-cadherin in human gastric adenocarcinoma. PLoS One 12:e0183692 (2017). PubMed: 28832634
View all Publications for this product

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Immunocytochemistry/ Immunofluorescence abreview for Anti-beta 1 Sodium Potassium ATPase antibody [M17-P5-F11]

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Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bone marrow derived mast cells)
Permeabilization
Yes - 0.1% Triton-X 100
Specification
Bone marrow derived mast cells
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
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Mrs. Nicole Corder

Verified customer

投稿 Jun 25 2018

Question

Do you have a mouse IgG2a ASCITES isotype avialable to use for ab2873? Also, can you give me a little more info on the purity of the antibody? Thank you

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Answer

Thank you for your enquiry. ab2873 (Sodium/Potassium ATPase Beta) is not affinity purified but comes as a PBS-diluted ascites containing 0.05% sodium azide. Please do not hesitate to contact me should you require further assistance.

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Question

For ab2871 and ab2873 - Customer would like to know if the epitope is extracellular or intracellular.

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Answer

Thank you for your patience. Purified lamb kidney beta sodium/potassium ATPase was used as the immunogen for both ab2871 and ab2873. Further characterization of the epitope region has not yet been performed. Therefore we are unable to determine the location of the epitope.

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