Name: Anti-ATP1B1 antibody [M17-P5-F11]
SKU: ab2873
Rating: 4 (1 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Key features and details

Mouse monoclonal [M17-P5-F11] to ATP1B1
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Human
Isotype: IgG2a

製品名

Anti-ATP1B1 antibody [M17-P5-F11]
ATP1B1 一次抗体製品一覧
製品の詳細

Mouse monoclonal [M17-P5-F11] to ATP1B1
由来種

Mouse
アプリケーション

適用あり: IHC-P, WB, ICC/IF, Flow Cytmore details
種交差性

交差種: Mouse, Human
交差が予測される動物種: Rabbit, Guinea pig, Chimpanzee, Cynomolgus monkey
免疫原

Full length native protein (purified) corresponding to Sheep ATP1B1. Purified lamb kidney sodium/potassium ATPase beta.
エピトープ

This antibody recognizes an epitope between amino acid residues 195-199 of sheep sodium/potassium ATPase beta 1.
特記事項

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファー

Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

M17-P5-F11
アイソタイプ

IgG2a
研究分野

Compatible Secondaries
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
Recombinant Protein
- Recombinant human ATP1B1 protein (Active) (ab222961)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab2873の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-P		1/200.
WB		1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
ICC/IF	(1)	1/100 - 1/1000.
Flow Cyt		1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

特記事項
IHC-P 1/200.
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
ICC/IF 1/100 - 1/1000.
Flow Cyt 1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

機能

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
組織特異性

Found in most tissues.
配列類似性

Belongs to the X(+)/potassium ATPases subunit beta family.
細胞内局在

Membrane.
Target information above from: UniProt accession P05026 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 481 Human
- Entrez Gene: 11931 Mouse
- Omim: 182330 Human
- SwissProt: Q9JM72 Guinea pig
- SwissProt: P05026 Human
- SwissProt: P14094 Mouse
- SwissProt: Q9TT37 Rabbit
- Unigene: 291196 Human
- Unigene: 4550 Mouse
see all
別名
- Adenosinetriphosphatase antibody
- AT1B1_HUMAN antibody
- ATP 1B antibody
- ATP1B antibody
- ATP1B1 antibody
- ATPase Na+/K+ transporting beta 1 polypeptide antibody
- Atpb 1 antibody
- ATPBS antibody
- Beta 1 subunit of Na(+) K(+) ATPase antibody
- Na K ATPase beta 1 polypeptide antibody
- Na+/K+ ATPase beta 1 subunit antibody
- Sodium/potassium dependent ATPase beta 1 subunit antibody
- Sodium/potassium transporting ATPase beta 1 chain antibody
- Sodium/potassium transporting ATPase subunit beta 1 antibody
- Sodium/potassium-dependent ATPase subunit beta-1 antibody
- Sodium/potassium-transporting ATPase subunit beta-1 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

IHC image of ab2873 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2873, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Immunocytochemistry/Immunofluorescence analysis of ATP1B1 shows staining in HeLa cells. ATP1B1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2873 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.
Western blot - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

All lanes : Anti-ATP1B1 antibody [M17-P5-F11] (ab2873) at 1/5000 dilution

Lane 1 : Human brain lysates
Lane 2 : Human liver lysates
Lane 3 : Human kidney lysates
Lane 4 : Mouse kidney lysates

Lysates/proteins at 25 µg per lane.

Secondary
All lanes : HRP-conjugated secondary antibody

Predicted band size: 35 kDa

Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate.
Immunocytochemistry/ Immunofluorescence - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Immunocytochemistry/Immunofluorescence analysis of ATP1B1 shows staining in MCF-7 cells. ATP1B1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2873 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Immunocytochemistry/Immunofluorescence analysis of ATP1B1 shows staining in U251 cells. ATP1B1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2873 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.
Flow Cytometry - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Overlay histogram showing HEK293 cells stained with ab2873 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2873, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase beta ab2873 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human liver tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase beta ab2873 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase beta ab2873 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheet download

Download

ab2873 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2873 は 15 報の論文で使用されています。

Nepal N et al. Mechanism of Na-K-ATPase Inhibition by PGE2 in Intestinal Epithelial Cells. Cells 10:N/A (2021). PubMed: 33805551
Luo J et al. Ad- and AAV8-mediated ABCA1 gene therapy in a murine model with retinal ischemia/reperfusion injuries. Mol Ther Methods Clin Dev 20:551-558 (2021). PubMed: 33665225
Nepal N et al. Unique Regulation of Na-K-ATPase during Growth and Maturation of Intestinal Epithelial Cells. Cells 8:N/A (2019). PubMed: 31208048
Scudieri P et al. Increased expression of ATP12A proton pump in cystic fibrosis airways. JCI Insight 3:N/A (2018). PubMed: 30333310
Schiapparelli P et al. NKCC1 Regulates Migration Ability of Glioblastoma Cells by Modulation of Actin Dynamics and Interacting with Cofilin. EBioMedicine 21:94-103 (2017). ICC/IF ; Human . PubMed: 28679472

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-3 of 3 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Bone marrow derived mast cells)

Permeabilization

Yes - 0.1% Triton-X 100

Specification

Bone marrow derived mast cells

Blocking step

BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 4°C

Fixative

Paraformaldehyde

Mrs. Nicole Corder

Verified customer

投稿 Jun 25 2018

Question

Do you have a mouse IgG2a ASCITES isotype avialable to use for ab2873? Also, can you give me a little more info on the purity of the antibody? Thank you

Abcam community

Verified customer

Asked on Sep 07 2005

Answer

Thank you for your enquiry. ab2873 (Sodium/Potassium ATPase Beta) is not affinity purified but comes as a PBS-diluted ascites containing 0.05% sodium azide. Please do not hesitate to contact me should you require further assistance.

Abcam Scientific Support

Answered on Sep 12 2005

Question

For ab2871 and ab2873 - Customer would like to know if the epitope is extracellular or intracellular.

Abcam community

Verified customer

Asked on Jul 09 2004

Answer

Thank you for your patience. Purified lamb kidney beta sodium/potassium ATPase was used as the immunogen for both ab2871 and ab2873. Further characterization of the epitope region has not yet been performed. Therefore we are unable to determine the location of the epitope.

Abcam Scientific Support

Answered on Jul 16 2004

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

エピトープ

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Compatible Secondaries

Conjugation kits

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

参考文献 (15)

レビューと Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-beta 1 Sodium Potassium ATPase antibody [M17-P5-F11]