Anti-ATP1B1 抗体 [464.8] (ab8344)

Reviews (1)Q&A (3)References (12)

Key features and details

  • Mouse monoclonal [464.8] to ATP1B1
  • Suitable for: Flow Cyt, WB
  • Reacts with: Rat, Cow, Dog, Human, Pig
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-ATP1B1 antibody [464.8]
    ATP1B1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [464.8] to ATP1B1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Flow Cyt, WBmore details

  • 種交差性

    交差種: Rat, Cow, Dog, Human, Pig

  • 免疫原

    corresponding to ATP1B1.

  • ポジティブ･コントロール

    • ab8344 gave a positive signal in human, mouse and rat kidney tissue lysates. In Flow Cytometry, this antibody gave a positive signal in methanol fixed/Tween permeabilised HepG2 cells.

  • 特記事項

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

     This product was previously labelled as beta 1 Sodium Potassium ATPase

     

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
WB 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa).

ターゲット情報

  • 機能

    This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.

  • 組織特異性

    Found in most tissues.

  • 配列類似性

    Belongs to the X(+)/potassium ATPases subunit beta family.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Adenosinetriphosphatase antibody
    • AT1B1_HUMAN antibody
    • ATP 1B antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • Atpb 1 antibody
    • ATPBS antibody
    • Beta 1 subunit of Na(+) K(+) ATPase antibody
    • Na K ATPase beta 1 polypeptide antibody
    • Na+/K+ ATPase beta 1 subunit antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • Sodium/potassium-dependent ATPase subunit beta-1 antibody
    • Sodium/potassium-transporting ATPase subunit beta-1 antibody
    see all

画像

  • Western blot - Anti-ATP1B1 antibody [464.8] (ab8344)
    Western blot - Anti-ATP1B1 antibody [464.8] (ab8344)
    Anti-ATP1B1 antibody [464.8] (ab8344) at 1/500 dilution + Human kidney tissue lysate - total protein (ab30203) at 20 µg

    Secondary
    Granzyme B overexpression lysate (ab94040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes
  • Flow Cytometry - Anti-ATP1B1 antibody [464.8] (ab8344)
    Flow Cytometry - Anti-ATP1B1 antibody [464.8] (ab8344)
    Overlay histogram showing HepG2 cells stained with ab8344 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8344, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献 (12)

ab8344 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab8344 は 12 報の論文で使用されています。

  • Wen X  et al. Gene-expression profile and localization of Na+/K(+)-ATPase in rat enamel organ cells. Eur J Oral Sci 122:21-6 (2014). PubMed: 24313748
  • Lu Q  et al. Ca²?/calmodulin-dependent protein kinase II contributes to hypoxic ischemic cell death in neonatal hippocampal slice cultures. PLoS One 8:e70750 (2013). WB ; Rat . PubMed: 23976956
  • Mroz MS  et al. Farnesoid X receptor agonists attenuate colonic epithelial secretory function and prevent experimental diarrhoea in vivo. Gut N/A:N/A (2013). WB ; Mouse . PubMed: 23916961
  • Lu Q  et al. Increased NADPH oxidase-derived superoxide is involved in the neuronal cell death induced by hypoxia-ischemia in neonatal hippocampal slice cultures. Free Radic Biol Med 53:1139-51 (2012). WB . PubMed: 22728269
  • Rafiqi FH  et al. Role of the WNK-activated SPAK kinase in regulating blood pressure. EMBO Mol Med 2:63-75 (2010). PubMed: 20091762
View all Publications for this product

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Western blot abreview for Anti-ATP1B1 antibody [464.8]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (enamel organ)
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Loading amount
15 µg
Specification
enamel organ
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

投稿 Feb 18 2013

Question

The human cells are isolated from blood, sonicated in Relaxin buffer containing protease inhibitors, then subjected to a saccharose gradient to separate the membrane. The protein is loaded on a gel. The membrane in blocked in Oddysey Blocking Buffer and the antibody is incubated overnight at 4C in PBST. No bands are observed.

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Answer

I can confirm that the laboratory has stopped using the Oddyssey blocking buffer as it gave a number of signal problems. We recommend to use 5% BSA or 5% milk, and both should work with the Oddyssey machine according to the lab. Can you please clarify whether the protocol of sucrose gradient is used with other proteins? I'm wondering if this step may be damaging the protein Sodium Potassium ATPase? If you still experience problems with the new blocking step please do not hesitate to contact me.

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Question

I am interesting in buying an antibody that is specific to Sodium potassium ATPase, that is specific to a peptide of this protein that lies within the cytoplasm. I believe that most commercial ATPase antibodies are specific to regions that are exposed on the cell surface. Do you know whether one of your antibodies actually target the internal face of the plasma membrane?

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Answer

Thank you for your enquiry. The epitope has not been mapped in the cases of ab7671 and ab8344 therefore we do not know which part of the protein is recognised by those antibodies. As the immunogen used to generate ab353 is purified Na,K-ATPase from rabbit renal outermedulla and the antibody is a polyclonal antibody the product will recognise a number of epitopes on the protein, some are likely to be at the plasma membrane portion intracellularly and some extracellularly. I hope this information helps, please do not hesitate to contact us again if you need more information,

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Question

We are working on CHO cell lines, doea this antibody recognize Sodium Potassium ATPase on CHO plasma membrane? Thanks for your time and reply in advance.

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Answer

This antibody has been shown to cross react with Cow, Dog, Human, Pig and Rat. I'm afraid that, to our knowledge, it has not yet been tested in other species, so I do not know if it will work with CHO cells.

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