Application
Western blot
Sample
Mouse Tissue lysate - other (brain)
Loading amount
30 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% milk in TBST
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/500
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~110 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 20 2010