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  1. Link

    atp1a3-antibody-xvif9-g10-ab2826.pdf

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Neuroscience Neurotransmission Receptors / Channels Potassium Channels
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Anti-ATP1A3 抗体 [XVIF9-G10] (ab2826)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (10)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Flow Cytometry - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

Key features and details

  • Mouse monoclonal [XVIF9-G10] to ATP1A3
  • Suitable for: Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog, Human, Pig, Shark
  • Isotype: IgG1

こちらの製品もご検討ください

一次抗体
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Anti-Calnexin antibody (ab10286)
タンパク質
Product image
Recombinant Human ATP1A3 protein (ab152203)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-ATP1A3 antibody [XVIF9-G10]
    ATP1A3 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [XVIF9-G10] to ATP1A3
  • 由来種

    Mouse
  • 特異性

    The immunogen used for this product shares 89% homology with ATP1A2. Cross-reactivity with this protein has not been confirmed experimentally
  • アプリケーション

    適用あり: Flow Cyt, ICC/IF, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog, Human, Pig, Shark
    交差が予測される動物種: Non human primates, Amphibian
  • 免疫原

    Full length native protein (purified) corresponding to Dog ATP1A3. Canine cardiac microsomes.

  • 特記事項

     This product was previously labelled as alpha 3 Sodium Potassium ATPase

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    XVIF9-G10
  • アイソタイプ

    IgG1
  • 研究分野

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Potassium Channels
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Sodium Channels
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human ATP1A3 protein (ab152203)

アプリケーション

Our Abpromise guarantee covers the use of ab2826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/50.
IHC-P 1/1000 - 1/10000.

Use at 1/1000 dilution for human, 1/10,000 dilution for mouse and rat

ターゲット情報

  • 機能

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • 関連疾患

    Dystonia 12
    Alternating hemiplegia of childhood 2
    Cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss
  • 配列類似性

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • 細胞内局在

    Cell membrane.
  • Target information above from: UniProt accession P13637 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 478 Human
    • Entrez Gene: 232975 Mouse
    • Entrez Gene: 24213 Rat
    • Omim: 182350 Human
    • SwissProt: P13637 Human
    • SwissProt: Q6PIC6 Mouse
    • SwissProt: P06687 Rat
    • Unigene: 515427 Human
    • Unigene: 44101 Mouse
    • Unigene: 87329 Rat
    see all
  • 別名

    • AHC2 antibody
    • Alpha(III) antibody
    • AT1A3_HUMAN antibody
    • Atp1a3 antibody
    • ATPase Na+/K+ transporting alpha 3 polypeptide antibody
    • DYT 12 antibody
    • DYT12 antibody
    • MGC13276 antibody
    • Na(+)/K(+) ATPase alpha(III) subunit antibody
    • Na(+)/K(+) ATPase alpha-3 subunit antibody
    • Na+/K+ ATPase 3 antibody
    • Na+/K+ ATPase alpha 3 subunit antibody
    • RDP antibody
    • Sodium potassium ATPase alpha 3 polypeptide antibody
    • Sodium pump 3 antibody
    • Sodium pump subunit alpha-3 antibody
    • Sodium/potassium transporting ATPase alpha 3 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-3 antibody
    see all

画像

  • Flow Cytometry - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Flow Cytometry - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Overlay histogram showing SH-SY5Y cells stained with ab2826 (red line). The cells were fixed with 4% paraformaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2826, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in C6 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in U251 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

    Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in HeLa cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (10)

    ab2826 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2826 は 10 報の論文で使用されています。

    • Fernández ÁF  et al. Interaction between the autophagy protein Beclin 1 and Na+,K+-ATPase during starvation, exercise, and ischemia. JCI Insight 5:N/A (2020). PubMed: 31941841
    • Mota MVB  et al. ATP Synthase Subunit Beta Immunostaining is Reduced in the Sclerotic Hippocampus of Epilepsy Patients. Cell Mol Neurobiol 39:149-160 (2019). PubMed: 30539418
    • Lüders KA  et al. Maintenance of high proteolipid protein level in adult central nervous system myelin is required to preserve the integrity of myelin and axons. Glia 67:634-649 (2019). PubMed: 30637801
    • Erwig MS  et al. Anillin facilitates septin assembly to prevent pathological outfoldings of central nervous system myelin. Elife 8:N/A (2019). PubMed: 30672734
    • Kawahata I  et al. Fatty Acid-Binding Protein 3 is Critical for a-Synuclein Uptake and MPP+-Induced Mitochondrial Dysfunction in Cultured Dopaminergic Neurons. Int J Mol Sci 20:N/A (2019). PubMed: 31661838
    • Huang H & Zhang W Bufalin induced apoptosis of bladder carcinoma cells through the inactivation of Na+K+-ATPase. Oncol Lett 16:3826-3832 (2018). PubMed: 30127995
    • Picton LD  et al. Sodium Pumps Mediate Activity-Dependent Changes in Mammalian Motor Networks. J Neurosci 37:906-921 (2017). PubMed: 28123025
    • Böhm S  et al. Peripherin-2 and Rom-1 have opposing effects on rod outer segment targeting of retinitis pigmentosa-linked peripherin-2 mutants. Sci Rep 7:2321 (2017). PubMed: 28539581
    • LaCrosse AL  et al. Contrasting the Role of xCT and GLT-1 Upregulation in the Ability of Ceftriaxone to Attenuate the Cue-Induced Reinstatement of Cocaine Seeking and Normalize AMPA Receptor Subunit Expression. J Neurosci 37:5809-5821 (2017). WB ; Rat . PubMed: 28495973
    • Datta P  et al. Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Proc Natl Acad Sci U S A 112:E4400-9 (2015). IF ; Mouse . PubMed: 26216965

    レビューと Q&A

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    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Frog Tissue lysate - whole (frog brain: forebrain, midbrain, hindbrain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    frog brain: forebrain, midbrain, hindbrain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 07 2019

    Immunocytochemistry abreview for Anti-ATP1A3 antibody [XVIF9-G10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (ALS patient iPSC derived Neurons)
    Permeabilization
    Yes - 0.1% Triton X - 5 min
    Specification
    ALS patient iPSC derived Neurons
    Blocking step
    BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Amit Chouhan

    Verified customer

    投稿 Mar 02 2017

    Question

    Dear Abcam Team,

    I am looking to perform some Immunohistochemistry experiments on rat myocardium and I was wondering whether you would recommend ab78798 or ab2826? We have not used any of these in our lab & I was looking for some guidance.

    Thanks for your advice,
    Yours,

    Read More

    Abcam community

    Verified customer

    Asked on Jan 17 2012

    Answer

    Thank you for your inquiry.

    As our Abpromise guarantees the species and the application stated on the datasheet, I recommend to order ab78798 if you plan to use IHC-P and ab2826 if you plan to use IHC-Fr.

    Both antibodies are tested in rat and therefore covered by our guarantee. Please follow this link to review the Abpromise guarantee:

    https://www.abcam.com/index.html?pageconfig=abpromise

    I would like to make you aware that matching secondary antibodies can be found using our search engine:

    https://www.abcam.com/index.html?pageconfig=productmap&cl=918

    And we also provide many IHC tailored products:

    https://www.abcam.com/index.html?pageconfig=resource&rid=12853

    I hope this information is helpful. Please do not hesitate to contact me again with more detailsabout the intendedexperiments if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Jan 17 2012

    Western blot abreview for Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (brain)
    Loading amount
    30 µg
    Specification
    brain
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 May 20 2010

    Western blot abreview for Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Brain cortex)
    Loading amount
    10 µg
    Specification
    Brain cortex
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 09 2009

    Western blot abreview for Anti-ATP1A3 antibody [XVIF9-G10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (primary cortical neurons)
    Gel Running Conditions
    Reduced Denaturing
    Treatment
    0-35 days in culture
    Specification
    primary cortical neurons
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 23 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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