Anti-ATP1A3 抗体 [XVIF9-G10] (ab2826)
Key features and details
- Mouse monoclonal [XVIF9-G10] to ATP1A3
- Suitable for: Flow Cyt, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog, Human, Pig, Shark
- Isotype: IgG1
製品の概要
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製品名
Anti-ATP1A3 antibody [XVIF9-G10]
ATP1A3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [XVIF9-G10] to ATP1A3 -
由来種
Mouse -
特異性
The immunogen used for this product shares 89% homology with ATP1A2. Cross-reactivity with this protein has not been confirmed experimentally -
アプリケーション
適用あり: Flow Cyt, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog, Human, Pig, Shark
交差が予測される動物種: Non human primates, Amphibian -
免疫原
Full length native protein (purified) corresponding to Dog ATP1A3. Canine cardiac microsomes.
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特記事項
This product was previously labelled as alpha 3 Sodium Potassium ATPase
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
XVIF9-G10 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ICC/IF | 1/50. | |
IHC-P | 1/1000 - 1/10000. Use at 1/1000 dilution for human, 1/10,000 dilution for mouse and rat |
ターゲット情報
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機能
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
関連疾患
Dystonia 12
Alternating hemiplegia of childhood 2
Cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss -
配列類似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 478 Human
- Entrez Gene: 232975 Mouse
- Entrez Gene: 24213 Rat
- Omim: 182350 Human
- SwissProt: P13637 Human
- SwissProt: Q6PIC6 Mouse
- SwissProt: P06687 Rat
- Unigene: 515427 Human
see all -
別名
- AHC2 antibody
- Alpha(III) antibody
- AT1A3_HUMAN antibody
see all
画像
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Overlay histogram showing SH-SY5Y cells stained with ab2826 (red line). The cells were fixed with 4% paraformaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2826, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in C6 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in U251 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in HeLa cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
プロトコール
参考文献 (10)
ab2826 は 10 報の論文で使用されています。
- Fernández ÁF et al. Interaction between the autophagy protein Beclin 1 and Na+,K+-ATPase during starvation, exercise, and ischemia. JCI Insight 5:N/A (2020). PubMed: 31941841
- Mota MVB et al. ATP Synthase Subunit Beta Immunostaining is Reduced in the Sclerotic Hippocampus of Epilepsy Patients. Cell Mol Neurobiol 39:149-160 (2019). PubMed: 30539418
- Lüders KA et al. Maintenance of high proteolipid protein level in adult central nervous system myelin is required to preserve the integrity of myelin and axons. Glia 67:634-649 (2019). PubMed: 30637801
- Erwig MS et al. Anillin facilitates septin assembly to prevent pathological outfoldings of central nervous system myelin. Elife 8:N/A (2019). PubMed: 30672734
- Kawahata I et al. Fatty Acid-Binding Protein 3 is Critical for a-Synuclein Uptake and MPP+-Induced Mitochondrial Dysfunction in Cultured Dopaminergic Neurons. Int J Mol Sci 20:N/A (2019). PubMed: 31661838
- Huang H & Zhang W Bufalin induced apoptosis of bladder carcinoma cells through the inactivation of Na+K+-ATPase. Oncol Lett 16:3826-3832 (2018). PubMed: 30127995
- Picton LD et al. Sodium Pumps Mediate Activity-Dependent Changes in Mammalian Motor Networks. J Neurosci 37:906-921 (2017). PubMed: 28123025
- Böhm S et al. Peripherin-2 and Rom-1 have opposing effects on rod outer segment targeting of retinitis pigmentosa-linked peripherin-2 mutants. Sci Rep 7:2321 (2017). PubMed: 28539581
- LaCrosse AL et al. Contrasting the Role of xCT and GLT-1 Upregulation in the Ability of Ceftriaxone to Attenuate the Cue-Induced Reinstatement of Cocaine Seeking and Normalize AMPA Receptor Subunit Expression. J Neurosci 37:5809-5821 (2017). WB ; Rat . PubMed: 28495973
- Datta P et al. Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Proc Natl Acad Sci U S A 112:E4400-9 (2015). IF ; Mouse . PubMed: 26216965