製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ATP11A
  • 由来種
    Rabbit
  • 特異性
    ab105351 is predicted to not cross react with AT11B. At least two isoforms of ATP11A are known to exist; this antibody will recognize both.
  • アプリケーション
    適用あり: ICC/IF, WB, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human ATP11A (N terminal).
    Database link: NP_115565

  • ポジティブ・コントロール
    • WB: K562 cell lysate ICC: K562 cells
  • 特記事項

    Previously labelled as AT11A. 

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab105351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 130 kDa.
ICC Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (Probable). May be involved in the uptake of farnesyltransferase inhibitor drugs, such as lonafarnib.
  • 配列類似性
    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.
  • 細胞内局在
    Cell membrane. Early endosome. Recycling endosome. Endoplasmic reticulum. Exit from the endoplasmic reticulum requires the presence of TMEM30A, but not TMEM30B. In the presence of TMEM30A, predominantly located in the plasma membrane.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    P-type ATPases, such as ATP11A, are phosphorylated in their intermediate state and drive uphill transport of ions across membranes.
  • 別名
    • AT11A antibody
    • AT11A_HUMAN antibody
    • Atp11a antibody
    • ATPase class VI type 11A antibody
    • ATPase IS antibody
    • ATPIH antibody
    • ATPIS antibody
    • P4-ATPase flippase complex alpha subunit ATP11A antibody
    • Probable phospholipid-transporting ATPase IH antibody
    see all

画像

  • Immunofluorescence of ATP11A in K562 cells with ab105351 at 20 ug/mL.

  • All lanes : Anti-ATP11A antibody (ab105351) at 1 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 130 kDa

  • ab105351, at 10 µg/ml, staining ATP11A in K562 cells by Immunocytochemistry.

参考文献

ab105351 has not yet been referenced specifically in any publications.

レビューと Q&A

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply and thank you for trying the mouse samples as well! I am very sorry that these3 antibodies did not perform as expected. I would be happy to offer you either a free of charge replacement for each antibody, or a credit or refund. For AT11A, we only have on other antibody in the catalog (ab117577) which has not yet been tested in mouse. For AT11B, we 2 other antibodies that are predicted to work in mouse, but also have not been tested with this species: ab82640 and ab117580. For AT11C, we have no other antibody available. I can offer you a credit or refund in this case or a replacement with an antibody to a different protein. In case of AT11A and AT11B, we are not able to guarantee the replacement antibodies for mouse (as they have not been tested), but I can give you a testing discount code for each replacement antibody. Alternatively, a different lot can be tried for ab105377 (AT11B). For the other 2 antibodies we currently have no vial in stock and I am not sure if the same or a new lot would come in next. Some more information on our testing discount program, should you decide to chose the antibodies that are not yet tested in mouse: For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. Please let me know how you would like to proceed and I'd be happy to arrange it for you. I look forward to hear back from you and resolve this case for you.

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Answer

Thank you for your phone call. I am sorry to hear that these antibodies are not providing satisfactory results so far. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results, as we discussed over the phone: 1) Since bovine samples have not been tested we cannot guarantee the antibodies for this species. However, mouse samples have been tested and you said you have mouse samples available to you. Thus, trying the antibodies on the mouse samples would be advisable to do. 2) Since high background was observed, using 5% milk for 2h (instead of 1% milk for 30 min) could help improve the signal and reduce the background. 3) Also, diluting the primary antibody in milk blocking solution can help as well. Should these suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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