ATP synthase Enzyme Activity Microplate Assay Kit (ab109714)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 6 hr
- Sample type: Cell culture extracts, Cell Lysate, Purified mitochondria, Suspension cells, Tissue, Tissue Extracts
製品の概要
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製品名
ATP synthase Enzyme Activity Microplate Assay Kit
ATP Synthase キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture extracts, Tissue, Suspension cells, Tissue Extracts, Cell Lysate, Purified mitochondria -
アッセイタイプ
Enzyme activity -
全工程の試験時間
6h 00m -
種交差性
交差種: Rat, Cow, Human
非交差種: Mouse -
製品の概要
ATP synthase Enzyme Activity Microplate Assay Kit ab109714 is used to determine the activity of ATP synthase (Complex V) in a human or rat sample.
The ATP synthase enzyme is immunocaptured within the wells of the microplate and the enzyme activity is measured by monitoring the decrease in absorbance at 340 nm. The conversion of ATP to ADP by ATP synthase is coupled to the oxidation reaction of NADH to NAD+ with a reduction in absorbance at 340 nm.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 ラベル 96 tests ATP synthase precoated Microtiter plate 1 unit Buffer Tube 1 1 x 10ml Detergent 1 x 1ml Phospholipids 1 x 6ml Reagent Mix 1 x 20ml -
研究分野
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別名
- Complex V
- F1F0
- F1F0 ATP synthase
see all -
参照データベース
- SwissProt: P25705 Human
- SwissProt: P10719 Rat
関連製品
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Positive Controls
画像
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Examples of activity/load relationships for cultured cell lysate, rat liver mitochondria, and bovine heart mitochondria.
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Functional study using ATP synthase Enzyme Activity Microplate Assay Kit (ab109714).
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Principle of ATP synthase Enzyme Activity Microplate Assay Kit (ab109714).
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Mitchondiral fractions from the heart tissue of Rats infected by S. pneumoniae, or given PBS sham control, were subjected to measurements of complex I-V activities. Complex I was measured with ab109721 (top left), Complex II + III were measured using ab109905 (top right), Complex IV was measured using ab109911 (bottom left) and Complex V was measured using ab109714 (bottom right).
Freshly isolated mitochondrial pellets were resuspened in PBS supplemented with 10% detergent provided in the kits. Protein concentrations of these mitochondrial lysates were estimated and 25 μg (for complex I, IV and V) or 100 μg (for complex II+III) mitochondrial protein was used per reaction. Enzyme activities were measured spectrophotometricly in triplicate and expressed as changes of absorbance per minute per mg protein.
データシートおよび資料
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Datasheet download
参考文献 (40)
ab109714 は 40 報の論文で使用されています。
- Kanakkanthara A et al. Repurposing Ceritinib Induces DNA Damage and Enhances PARP Inhibitor Responses in High-Grade Serous Ovarian Carcinoma. Cancer Res 82:307-319 (2022). PubMed: 34810199
- Rajab BS et al. Differential remodelling of mitochondrial subpopulations and mitochondrial dysfunction are a feature of early stage diabetes. Sci Rep 12:978 (2022). PubMed: 35046471
- Zeng Z et al. Acetylation of Atp5f1c Mediates Cardiomyocyte Senescence via Metabolic Dysfunction in Radiation-Induced Heart Damage. Oxid Med Cell Longev 2022:4155565 (2022). PubMed: 36160705
- Lı N et al. Impact of ATP synthase/coupling factor 6 in hypoxic pulmonary arterial hypertension: An experimental rat model. Turk J Med Sci 52:1468-1477 (2022). PubMed: 36422496
- Chaudhary S et al. Mitochondrial complex II and V activity is enhanced in pediatric acute myeloid leukemia. Am J Blood Res 11:534-543 (2021). PubMed: 34824886