Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Head)
Loading amount
40 µg
Specification
Head
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5% milk
Secondary antibody
Name
Non-Abcam antibody was used: Licoer anti Rab-green
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/12000
Detection
Detection method
Licor machine
Exposure
1000 hour(s), 40 minute(s) and 39 second(s)
Bands
Specific: 122 kDa
Additional data
Abcam response
We wish to thank the reviewer for providing this Abreview for Drosphila melanogaster
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 02 2012