Name: Anti-ATP citrate lyase antibody [EP704Y]
Brand: RabMAb
SKU: ab40793
Rating: 4 (2 reviews)

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Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP704Y] to ATP citrate lyase
Suitable for: IHC-P, ICC/IF, WB, Flow Cyt, IP
Knockout validated
Reacts with: Mouse, Rat, Human

製品名

Anti-ATP citrate lyase antibody [EP704Y]
ATP citrate lyase 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EP704Y] to ATP citrate lyase
由来種

Rabbit
特異性

ab40793 recognises ATP citrate lyase (ACL). The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

免疫原

Synthetic peptide within Human ATP citrate lyase aa 1050 to the C-terminus (C terminal). The exact sequence is proprietary.
(Peptide available as ab207504)
ポジティブ･コントロール
- WB: HeLa cell lysate; NIH/3T3; rat lung; C6 lysates. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. IP: Jurkat cell lysate; HeLa. IHC: Human clear cell carcinoma of kidney
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EP704Y
アイソタイプ

IgG
研究分野

Alternative Versions
Compatible Secondaries
Immunizing Peptide (Blocking)
- ATP citrate lyase peptide (ab207504)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human ATP citrate lyase protein (His tag) (ab235007)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab40793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Mouse Rat Human
All applications	Common marmoset

アプリケーション	Abreviews	特記事項
IHC-P		Use at an assay dependent concentration.
ICC/IF		1/50.
WB	(2)	1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 122 kDa).Can be blocked with ATP citrate lyase peptide (ab207504). For unpurified use at 1/1000 - 1/5000.
Flow Cyt		1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100.
IP		1/20.

特記事項
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50.
WB 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 122 kDa).Can be blocked with ATP citrate lyase peptide (ab207504). For unpurified use at 1/1000 - 1/5000.
Flow Cyt 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100.
IP 1/20.

機能

ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
配列類似性

In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
Contains 1 ATP-grasp domain.
翻訳後修飾

ISGylated.
Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
細胞内局在

Cytoplasm.
Target information above from: UniProt accession P53396 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 47 Human
- Entrez Gene: 104112 Mouse
- Entrez Gene: 24159 Rat
- Omim: 108728 Human
- SwissProt: P53396 Human
- SwissProt: Q91V92 Mouse
- SwissProt: P16638 Rat
- Unigene: 387567 Human
- Unigene: 282039 Mouse
- Unigene: 29771 Rat
see all
別名
- ACL antibody
- Acly antibody
- ACLY_HUMAN antibody
- ATP citrate (pro-S) lyase antibody
- ATP citrate lyase antibody
- ATP citrate synthase antibody
- ATP-citrate (pro-S-)-lyase antibody
- ATP-citrate synthase antibody
- ATPcitrate synthase antibody
- ATPCL antibody
- Citrate cleavage enzyme antibody
- CLATP antibody
- OTTHUMP00000164773 antibody
see all

Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ATP citrate lyase knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)

Lanes 1 - 3: Merged signal (red and green). Green - ab40793 observed at 125 kDa. Red - loading control, ab9484, observed at 37 kDa.

Unpurified ab40793 was shown to specifically react with ATP citrate lyase in wild-type HAP1 cells as signal was lost in ATP citrate lyase knockout cells. Wild-type and ATP citrate lyase knockout samples were subjected to SDS-PAGE. ab40793 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling ATP citrate lyase with purified ab40793 at 1/50. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human clear cell carcinoma of kidney tissue sections labeling ATP citrate lyase with Purified ab40793 at 1:100 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Flow Cytometry - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ATP citrate lyase with purified ab40793 at 1:30 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

All lanes : Anti-ATP citrate lyase antibody [EP704Y] (ab40793) at 1/50000 dilution (purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 122 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

ab40793 (purified) at 1:20 dilution (1.5μg) immunoprecipitating ATP citrate lyase in HeLa whole cell lysate.

Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate,10μg
Lane 2 (+): ab40793 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40793 in HeLa whole cell lysate.

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

All lanes : Anti-ATP citrate lyase antibody [EP704Y] (ab40793) at 1/10000 dilution (purified)

Lane 1 : Rat lung lysates
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 122 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

Anti-ATP citrate lyase antibody [EP704Y] (ab40793) at 1/5000 dilution (unpurified) + HeLa cell lysate

Predicted band size: 122 kDa
Observed band size: 122 kDa
Flow Cytometry - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

Overlay histogram showing HeLa cells stained with unpurified ab40793 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40793, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Immunoprecipitation - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

Unpurified ab40793 at 1/40 immunoprecipitating ATP citrate lyase in HeLa (human cervix adenocarcinoma) whole cell lysate.

Lane 1 (input): HeLa (human cervix adenocarcinoma) whole cell lysate 10μg

Lane 2 (+): ab40793 + HeLa (human cervix adenocarcinoma) whole cell lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40793 in HeLa (human cervix adenocarcinoma) whole cell lysate

For western blotting, ab40793 at 1/1000 dilution and ab131366 VeriBlot for IP (HRP) was used for detection at 1/10000.

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.
Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab40793 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab40793 は 26 報の論文で使用されています。

Jin F et al. HIF-1a-induced miR-23a~27a~24 cluster promotes colorectal cancer progression via reprogramming metabolism. Cancer Lett 440-441:211-222 (2019). PubMed: 30393198
Shi Z et al. Silibinin inhibits endometrial carcinoma via blocking pathways of STAT3 activation and SREBP1-mediated lipid accumulation. Life Sci 217:70-80 (2019). PubMed: 30452972
Xue D et al. Quantitative proteomic analysis of sperm in unexplained recurrent pregnancy loss. Reprod Biol Endocrinol 17:52 (2019). PubMed: 31288842
Favara DM et al. ADGRL4/ELTD1 Silencing in Endothelial Cells Induces ACLY and SLC25A1 and Alters the Cellular Metabolic Profile. Metabolites 9:N/A (2019). PubMed: 31775252
Chen Y et al. ATP-citrate lyase is an epigenetic regulator to promote obesity-related kidney injury. FASEB J 33:9602-9615 (2019). PubMed: 31150280

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-5 of 5 Abreviews or Q&A

Application

Western blot

Loading amount

80 µg

Gel Running Conditions

Reduced Denaturing (10% SDS-PAGE)

Sample

Marmoset (common) Tissue lysate - whole (brain)

Specification

brain

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

投稿 Jun 05 2014

Question

What's the concentration?

Abcam community

Verified customer

Asked on Nov 28 2012

Answer

Vielen Dank für Ihren Anruf.

Leider ermitteln wir für diesen Antikörper die Konzentration nicht, so dass ich Ihnen leider in diesem Fall nicht weiterhelfen kann.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Abcam Scientific Support

Answered on Nov 28 2012

Application

Western blot

Sample

Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Head)

Loading amount

40 µg

Specification

Head

Gel Running Conditions

Reduced Denaturing (12%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

投稿 Oct 02 2012

Question

How are you?

I am almost ready to submit the blots showing it worked. Whatexactlyshould I send you so I could get two new antibodies for free?

Abcam community

Verified customer

Asked on Aug 24 2012

Answer

Please log into your Abcam account, and than open the datasheet for the antibody you would like to write a review about. On top of the datasheet, you will find five tags : Datasheet, specific references, Abreview, Scientific Support, Protocols.

You have to click (you guessed it) on the Abreview section, and just follow the Submit an Abreviewlink.

You will find a drop- down menu which is pretty self explainingfrom which you have to choose your used application, species, blocking conditions, etc. An image is always welcome as well, so feel free to upload one.

However, to inform us that this is a 100% Test-discount Review, please add one of the discount codes into the "additional information "section at the end of the review. Submit it, and voila, you are ready to order anantibody for free!

Abcam Scientific Support

Answered on Aug 24 2012

Question

,

Thank you very much!

The first antibody is :

Anti-Histone H4 (acetyl K12) antibody (ab61238)
Which appears to also work on WB Drosophila.

The second is:

Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
Which appears to work on WB in Drosophila - I want to run more test to make sure.

What does the discount means exactly? How can I use it?

Bw

Abcam community

Verified customer

Asked on May 08 2012

Answer

Expiration date: 08. September 2012

ab40793 -DISCOUNT CODE: xxxx

ab61238- DISCOUNT CODE: xxxx

I am very pleased to hear you accepted our offer and tested ab40793 und ab61238 in drosophila. This code will give you: 1 freeprimary antibody per codebefore the expiration date. To redeem this offer, please submit an Abreview fordrosophila for each antibody and include the aprobriatecode in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Abcam Scientific Support

Answered on May 08 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

製品の概要

製品名

製品の詳細

由来種

特異性

Tested Applications & Species

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Immunizing Peptide (Blocking)

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (26)

レビューと Q&A

Western blot abreview for Anti-ATP citrate lyase antibody [EP704Y]

Western blot abreview for Anti-ATP citrate lyase antibody [EP704Y]