Anti-ATG16L1 抗体 [5H9A11] (ab233796)
Key features and details
- Mouse monoclonal [5H9A11] to ATG16L1
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-ATG16L1 antibody [5H9A11]
ATG16L1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5H9A11] to ATG16L1 -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human ATG16L1 aa 11-257. Expressed in E.coli.
Database link: Q676U5 -
ポジティブ・コントロール
- WB: Recombinant human ATG16L1 (aa 11-257) protein; ATG16L1 (aa 11-257)-hIgG-Fc-transfected HEK-293 cell lysate; HeLa, Raji, PANC-1, Jurkat, Daudi, PC-12, HepG2, HEK-293 and NIH/3T3 cell lysates, Wild-type THP-1 cell lysate, Wild type HeLa cell lysate. Flow cyt: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
5H9A11 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab233796の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 68 kDa.
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Flow Cyt |
1/200 - 1/400.
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特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 68 kDa. |
Flow Cyt
1/200 - 1/400. |
ターゲット情報
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機能
Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane. -
関連疾患
Inflammatory bowel disease 10 -
配列類似性
Belongs to the WD repeat ATG16 family.
Contains 7 WD repeats. -
翻訳後修飾
Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli. -
細胞内局在
Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme. - Information by UniProt
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参照データベース
- Entrez Gene: 55054 Human
- Entrez Gene: 77040 Mouse
- Entrez Gene: 363278 Rat
- Omim: 610767 Human
- SwissProt: Q676U5 Human
- SwissProt: Q8C0J2 Mouse
- Unigene: 529322 Human
- Unigene: 272972 Mouse
see all -
製品の状態
There are 4 isoforms produced by alternative splicing. -
別名
- A16L1_HUMAN antibody
- APG16 like 1 antibody
- APG16-like 1 antibody
see all
画像
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All lanes : Anti-ATG16L1 antibody [5H9A11] (ab233796) at 1/500 dilution
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : ATG16L1 knockout THP-1 cell lysate
Lane 3 : Wild type HeLa cell lysate
Lane 4 : ATG16L1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68,70 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-ATG16L1 antibody [5H9A11] staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab233796 was shown to bind specifically to ATG16L1. A band was observed at 68/70 kDa in wild-type THP-1 cell lysates with no signal observed at this size in ATG16L1 knockout cell line ab277834 (knockout cell lysate ab278184). To generate this image, wild-type and ATG16L1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
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All lanes : Anti-ATG16L1 antibody [5H9A11] (ab233796) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ATG16L1 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDaLanes 1- 4: Merged signal (red and green). Green - ab233796 observed at 68 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.
ab233796 was shown to react with ATG16L1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265263 (knockout cell lysate ab256842) was used. Wild-type HeLa and ATG16L1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab233796 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-ATG16L1 antibody [5H9A11] (ab233796) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ATG16L1 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68,72 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab233796 observed at 68 and 72 kDa. Red - loading control ab181602 observed at 37 kDa.
ab233796 Anti-ATG16L1 antibody [5H9A11] was shown to specifically react with ATG16L1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261773 (knockout cell lysate ab256844) was used. Wild-type and ATG16L1 knockout samples were subjected to SDS-PAGE. ab233796 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-ATG16L1 antibody [5H9A11] (ab233796) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ATG16L1 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68,72 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab233796 observed at 68 and 72 kDa. Red - loading control ab181602 observed at 37 kDa.
ab233796 Anti-ATG16L1 antibody [5H9A11] was shown to specifically react with ATG16L1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261772 (knockout cell lysate ab256843) was used. Wild-type and ATG16L1 knockout samples were subjected to SDS-PAGE. ab233796 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Anti-ATG16L1 antibody [5H9A11] (ab233796) at 1/500 dilution + Recombinant human ATG16L1 (aa 11-257) protein
Predicted band size: 68 kDaExpected MW is 56 kDa.
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All lanes : Anti-ATG16L1 antibody [5H9A11] (ab233796) at 1/500 dilution
Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : ATG16L1 (aa 11-257)-hIgG-Fc-transfected HEK-293 cell lysate
Predicted band size: 68 kDa -
All lanes : Anti-ATG16L1 antibody [5H9A11] (ab233796) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) cell lysate
Lane 3 : PANC-1 (human pancreatic epithelial cancinoma cell line) cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 7 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 8 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Predicted band size: 68 kDa -
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ATG16L1 with ab233796 at 1/200 dilution (green) compared with a negative control (red).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab233796 は論文での使用が確認できていません。