製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ATG10
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow
  • 免疫原

    Synthetic peptide corresponding to Human ATG10 aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90636)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: DU 145; Raji.
  • 特記事項

    Previously labelled as Apg10. 

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

ターゲット情報

  • 機能
    E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.
  • 配列類似性
    Belongs to the ATG10 family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apg 10 antibody
    • APG 10L antibody
    • APG10 antibody
    • APG10 autophagy 10 like antibody
    • APG10 like antibody
    • APG10, S. cerevisiae, homolog of antibody
    • APG10-like antibody
    • APG10L antibody
    • ATG 10 antibody
    • ATG10 antibody
    • ATG10 autophagy related 10 homolog (S. cerevisiae) antibody
    • ATG10 autophagy related 10 homolog antibody
    • ATG10_HUMAN antibody
    • autophagy 10, S. cerevisiae, homolog of antibody
    • Autophagy related protein 10 antibody
    • Autophagy-related protein 10 antibody
    • DKFZP586I0418 antibody
    • FLJ13954 antibody
    • pp12616 antibody
    • Ubiquitin-like-conjugating enzyme ATG10 antibody
    see all

画像

  • All lanes : Anti-ATG10 antibody (ab79316) at 1 µg/ml

    Lane 1 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa
    Additional bands at: 34 kDa, 37 kDa, 42 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes

プロトコール

参考文献

ab79316 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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