Anti-ATF2 抗体 (ab809)

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Western blot - Anti-ATF2 antibody (ab809)

    Key features and details

    • Goat polyclonal to ATF2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-ATF2 (phospho T71) antibody [E268] (ab32019)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-ATF2 antibody
      ATF2 一次抗体 製品一覧

    • 製品の詳細

      Goat polyclonal to ATF2

    • 由来種

      Goat

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Rat, Cow, Dog

    • 免疫原

      Synthetic peptide corresponding to Human ATF2 aa 492-505 (C terminal).
      Sequence:

      C-IVMAPSSQSQPSGS


      (Peptide available as ab22789)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • WB: HepG2, K562 and HeLa nuclear lysates.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.05% Tris, 0.5% BSA
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab809 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use a concentration of 3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).

    Primary incubation 1 hour at room temperature.

    ターゲット情報

    • 機能

      Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.

    • 組織特異性

      Abundant expression seen in the brain.

    • 配列類似性

      Belongs to the bZIP family. ATF subfamily.
      Contains 1 bZIP domain.
      Contains 1 C2H2-type zinc finger.

    • 翻訳後修飾

      Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.

    • 細胞内局在

      Nucleus.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Activating transcription factor 2 antibody
        • Activating transcription factor 2 splice variant ATF2 var2 antibody
        • ATF 2 antibody
        • Atf-2 antibody
        • Atf2 antibody
        • ATF2 protein antibody
        • ATF2_HUMAN antibody
        • cAMP Response Element Binding Protein 2 antibody
        • cAMP response element binding protein CRE BP1 antibody
        • cAMP response element-binding protein CRE-BP1 antibody
        • cAMP responsive element binding protein 2, formerly antibody
        • cAMP-dependent transcription factor ATF-2 antibody
        • cAMP-responsive element-binding protein 2 antibody
        • CRE BP1 antibody
        • CRE-BP antibody
        • CREB 2 antibody
        • CREB-2 antibody
        • CREB2 antibody
        • CREBP1 antibody
        • Cyclic AMP dependent transcription factor ATF 2 antibody
        • Cyclic AMP-dependent transcription factor ATF-2 antibody
        • Cyclic AMP-responsive element-binding protein 2 antibody
        • D130078H02Rik antibody
        • D18875 antibody
        • HB 16 antibody
        • HB16 antibody
        • Histone acetyltransferase ATF2 antibody
        • MGC105211 antibody
        • MGC105222 antibody
        • MGC111558 antibody
        • MGC142504 antibody
        • mXBP antibody
        • MXBP protein antibody
        • Tg(Gzma-Klra1)7Wum antibody
        • TREB 7 antibody
        • TREB7 antibody
        see all

      画像

      • Western blot - Anti-ATF2 antibody (ab809)
        Western blot - Anti-ATF2 antibody (ab809)
        All lanes : Anti-ATF2 antibody (ab809) at 1 µg/ml

        Lane 1 : HepG2 nuclear lysate in RIPA buffer
        Lane 2 : K562 nuclear lysate in RIPA buffer
        Lane 3 : HeLa nuclear lysate in RIPA buffer

        Lysates/proteins at 35 µg per lane.

        Predicted band size: 54 kDa



        Detected by chemiluminescense.

      参考文献 (1)

      ab809 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab809 は 1 報の論文で使用されています。

      • Karim AS  et al. Nox2 is a mediator of ischemia reperfusion injury. Am J Transplant 15:2888-99 (2015). PubMed: 26104383

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