製品の概要
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製品名Anti-ATF2 antibody
ATF2 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to ATF2
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由来種Goat
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アプリケーション適用あり: WBmore details
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種交差性交差種: Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide: IVMAPSSQSQPSGS, corresponding to C terminal amino acids 492-505 of Human ATF2.
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ポジティブ･コントロール
- HeLa cell lysates
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human ATF2 peptide (ab22789). Overnight incubation is recommended. |
ターゲット情報
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機能Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
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組織特異性Abundant expression seen in the brain.
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配列類似性Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. -
翻訳後修飾Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 1386 Human
- Entrez Gene: 81647 Rat
- Omim: 123811 Human
- SwissProt: P15336 Human
- SwissProt: Q00969 Rat
- Unigene: 592510 Human
- Unigene: 9825 Rat
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別名
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
see all
画像
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Western blot - Anti-ATF2 antibody (ab809)staining (2
staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.µ g/ml) of HeLa lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.
プロトコール
参考文献
This product has been referenced in:
- Karim AS et al. Nox2 is a mediator of ischemia reperfusion injury. Am J Transplant 15:2888-99 (2015). Read more (PubMed: 26104383) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"