Anti-ATF2 抗体 (ab809)
Key features and details
- Goat polyclonal to ATF2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-ATF2 antibody
ATF2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ATF2 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Cow, Dog -
免疫原
Synthetic peptide corresponding to Human ATF2 aa 492-505 (C terminal).
Sequence:C-IVMAPSSQSQPSGS
(Peptide available asab22789) -
ポジティブ・コントロール
- WB: HepG2, K562 and HeLa nuclear lysates.
-
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | Use a concentration of 3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa). Primary incubation 1 hour at room temperature. |
ターゲット情報
-
機能
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. -
組織特異性
Abundant expression seen in the brain. -
配列類似性
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. -
翻訳後修飾
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 509827 Cow
- Entrez Gene: 1386 Human
- Entrez Gene: 81647 Rat
- Omim: 123811 Human
- SwissProt: P15336 Human
- SwissProt: Q00969 Rat
- Unigene: 592510 Human
- Unigene: 9825 Rat
-
別名
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
see all
画像
-
All lanes : Anti-ATF2 antibody (ab809) at 1 µg/ml
Lane 1 : HepG2 nuclear lysate in RIPA buffer
Lane 2 : K562 nuclear lysate in RIPA buffer
Lane 3 : HeLa nuclear lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 54 kDaDetected by chemiluminescense.
データシートおよび資料
参考文献 (1)
ab809 は 1 報の論文で使用されています。
- Karim AS et al. Nox2 is a mediator of ischemia reperfusion injury. Am J Transplant 15:2888-99 (2015). PubMed: 26104383
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com