Anti-ATF2 抗体 (ab809)
製品の概要
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製品名
Anti-ATF2 antibody
ATF2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ATF2 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Cow, Dog
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免疫原
Synthetic peptide corresponding to Human ATF2 aa 492-505 (C terminal).
Sequence:C-IVMAPSSQSQPSGS
(Peptide available asab22789) -
ポジティブ･コントロール
- WB: HepG2, K562 and HeLa nuclear lysates.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human ATF2 peptide (ab22789). Primary incubation 1 hour at room temperature. |
ターゲット情報
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機能
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. -
組織特異性
Abundant expression seen in the brain. -
配列類似性
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. -
翻訳後修飾
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 509827 Cow
- Entrez Gene: 1386 Human
- Entrez Gene: 81647 Rat
- Omim: 123811 Human
- SwissProt: P15336 Human
- SwissProt: Q00969 Rat
- Unigene: 592510 Human
- Unigene: 9825 Rat
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別名
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
see all
画像
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Western blot - Anti-ATF2 antibody (ab809)All lanes : Anti-ATF2 antibody (ab809) at 1 µg/ml
Lane 1 : HepG2 nuclear lysate in RIPA buffer
Lane 2 : K562 nuclear lysate in RIPA buffer
Lane 3 : HeLa nuclear lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 54 kDaDetected by chemiluminescense.
プロトコール
データシートおよび資料
参考文献
This product has been referenced in:
- Karim AS et al. Nox2 is a mediator of ischemia reperfusion injury. Am J Transplant 15:2888-99 (2015). Read more (PubMed: 26104383) »
