製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ATF-4
  • 由来種
    Rabbit
  • 特異性
    Antibody detects endogenous levels of ATF-4 protein around Serine 245.
  • アプリケーション
    適用あり: ICC/IF, IHC-Fr, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human ATF-4.
    Database link: P18848

  • ポジティブ・コントロール
    • Breast carcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab31390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 21933012Fixed with methanol
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

ターゲット情報

  • 機能
    Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
  • 配列類似性
    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • 細胞内局在
    Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activating transcription factor 4 antibody
    • ATF 4 antibody
    • ATF4 antibody
    • ATF4 protein antibody
    • ATF4_HUMAN antibody
    • cAMP-dependent transcription factor ATF-4 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • Cyclic AMP dependent transcription factor ATF 4 antibody
    • Cyclic AMP response element binding protein 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-4 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • DNA binding protein TAXREB67 antibody
    • DNA-binding protein TAXREB67 antibody
    • Tax Responsive Enhancer Element B67 antibody
    • Tax-responsive enhancer element-binding protein 67 antibody
    • TaxREB67 antibody
    • TXREB antibody
    see all

画像

  • ab31390 at a 1:50-1:100 dilution staining ATF-4 in human breast carcinoma tissue, using Immunohistochemistry, Paraffin Embedded Tissue.

    Left image : Un-treated.

    Right image : Antibody pre-incubated with synthesized peptide.

参考文献

This product has been referenced in:
  • Zhu H  et al. Activating transcription factor 4 promotes esophageal squamous cell carcinoma invasion and metastasis in mice and is associated with poor prognosis in human patients. PLoS One 9:e103882 (2014). IHC-P ; Human . Read more (PubMed: 25078779) »
  • Lenna S  et al. HLA-B35 and dsRNA induce endothelin-1 via activation of ATF4 in human microvascular endothelial cells. PLoS One 8:e56123 (2013). IHC-P ; Human . Read more (PubMed: 23441162) »

See all 5 Publications for this product

レビューと Q&A

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary rat cortical neurons)
Permeabilization
Yes - 0.1% Triton-x
Specification
Primary rat cortical neurons
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 30 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Lung)
Specification
Lung
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Dr. Carl Atkinson

Verified customer

投稿 Apr 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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