製品の概要

  • 製品名
    Aspartate Aminotransferase Activity Assay Kit (Fluorometric)
    Aspartate Aminotransferase キット 製品一覧
  • 検出方法
    Fluorescent
  • サンプルの種類
    Serum, Other biological fluids
  • アッセイタイプ
    Enzyme activity
  • 検出感度
    2 mU/ml
  • 種交差性
    交差種: Mammals, Other species
  • 製品の概要

    Abcam's Aspartate Aminotransferase Activity Assay Kit (ab138878) provides a quick and sensitive method for the measurement of AST (Aspartate aminotransferase) in various biological samples. AST catalyzes the reaction of aspartate and α-ketoglutarate to oxaloacetate and glutamate:

    Aspartate (Asp) + α-ketoglutarate ? oxaloacetate + glutamate (Glu)

    The product L-glutamate is measured by the generation of a red fluorescent product through an enzyme coupled reaction cycle. The signal can be read by a fluorescence microplate reader at Ex/Em = 530-570 nm/590-600 nm (optimal Ex/Em = 540 nm/590 nm). ab138878 can detect as little as 2 mU/mL AST in a 100 µL reaction volume. The assay is robust, and can be readily adapted for a wide variety of applications.

  • 特記事項

    Aspartate aminotransferase (AST), also called Glutamic Oxaloacetic transaminase (GOT), is a member of transferase family. It catalyzes the reversible transfer of an α-amino group between aspartate and glutamate, and is an important enzyme in amino acid metabolism. AST is found in many body tissues such as liver, heart, muscle, kidneys, brain. In healthy subjects, serum AST levels are low. However, when cells are damaged, such as acute and chronic hepatitis, obstructive jaundice, carcinoma of liver, myocardial infarction, AST may leak into the blood stream and the AST levels are significantly elevated. Therefore, determination of serum AST level has great clinical and diagnostic significance.

  • 試験プラットフォーム
    Microplate reader

製品の特性

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  • AST dose response was measured with ab138878 in a 96-well black plate using a fluorescence microplate reader. As low as 2 mU/ml AST can be detected with 60 min incubation (n=3) at room temperature.

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参考文献

ab138878 has not yet been referenced specifically in any publications.

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