製品の概要

  • 製品名
    Aspartate Aminotransferase Activity Assay Kit
    Aspartate Aminotransferase キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプ
    Enzyme activity
  • 検出感度
    10 mU/well
  • 全工程の試験時間
    1h 00m
  • 製品の概要

    Aspartate Aminotransferase Activity Assay Kit (AST assay kit) ab105135 provides a rapid, simple, sensitive and reliable assay for AST activity.


    In the AST assay protocol, an amino group is transferred from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that converts a nearly colorless probe to color (λmax = 450 nm).


    The AST assay has a detection limit of 10 mU per well.


    AST assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - incubate at 37ºC and analyze with microplate reader after 10 mins and then again after 60 mins

  • 特記事項

    Aspartate aminotransferase (AST), also known as Glutamate-oxaloacetate transaminase (GOT) is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + alpha-Ketoglutarate = Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L).

  • 試験プラットフォーム
    Microplate reader

製品の特性

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  • AST activity measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1 = 2 min; T2 = 60 min).

  • AST activity measured in human biological fluids showing activity (mU) per mL of tested sample, (T1 = 2 min; T2 = 60 min) with background signals subtracted.

  • AST activity measured in cell culture medium and control medium, background signal subtracted. T1 = 2 min; T2 = 60 min.

  • AST activity measured in cell lysates (T1 = 2 min; T2 = 60 min; duplicates (+/- SD), background signal subtracted).

  • Standard curve (60 minutes incubation): mean of duplicates (+/- SD) with background reads subtracted.

  • Example of Standard Curve and Positive control detection using ab105135.

プロトコール

参考文献

This product has been referenced in:
  • da Silva-Candal A  et al. Clinical validation of blood/brain glutamate grabbing in acute ischemic stroke. Ann Neurol 84:260-273 (2018). Read more (PubMed: 30014516) »
  • Alam MF  et al. Thymoquinone Ameliorates Doxorubicin-Induced Cardiotoxicity in Swiss Albino Mice by Modulating Oxidative Damage and Cellular Inflammation. Cardiol Res Pract 2018:1483041 (2018). Read more (PubMed: 29805796) »
See all 9 Publications for this product

レビューと Q&A

1-2 of 2 Abreviews or Q&A

Answer



It is recommended to measure standard curve at both times 10 mins and 60 mins. Then use the difference in Abs at 60min - Abs at 10min to plot the Std curve (OD on Y axis versus Glutamate nmol on the X axis).

Please let me know if you have any further questions.

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Answer

The identity of all the kit components is proprietary information. The mechanism of the reaction is exactly as mentioned on the datasheet. The substrate is a sequence the AST and the other enzymes in the provided mix will act upon. The probe will interact with the glutamate in the presence of the enzymes and produce a coloured product which can be measured.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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