Application
Western blot
Sample
Mouse Cell lysate - whole cell (Bone-marrow derived dendritic cells)
Loading amount
70 µg
Specification
Bone-marrow derived dendritic cells
Gel Running Conditions
Reduced Denaturing (8% SDS gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL+
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 155 kDa Non-specific: 110, 40 kDa
Additional data
Additional Notes
12 min. exposure or increased/decreased dilutions of 1st and 2nd antibody can be used to detect ASK1 in even lower amount of total cell lysates.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Caglar Cekic
Verified customer
投稿 May 13 2009