Arylamine N-acetyltransferase Activity Assay Kit (Fluorometric) (ab284533)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Sample type: Purified protein
製品の概要
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製品名
Arylamine N-acetyltransferase Activity Assay Kit (Fluorometric) -
検出方法
Fluorescent -
サンプルの種類
Purified protein -
アッセイタイプ
Enzyme activity (quantitative) -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rat, Human -
製品の概要
Arylamine N-acetyltransferase Activity Assay Kit (Fluorometric) (ab284533) (K2031) is a simple, one step plate based assay that measures both NAT1 and NAT2 activity in tissue samples, S9 fractions, recombinant enzyme or purified protein samples.
This kit can detect as low as 1 µU in samples.
General range: 0-500 pmol/well of acetylated standard.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K2031 Arylamine N-acetyltransferase Activity Assay Kit (Fluorometric). K2031-100 is the same size as the 100 test size of ab284533.
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アプリケーション
適用あり: Enzyme activity assaymore details -
試験プラットフォーム
Microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Acetylated Standard 1 x 50µl DTT 1 x 100µl NAT Assay Buffer 1 x 25ml NAT Positive Control 1 vial NAT Substrate I 1 x 200µl NAT Substrate II 2 vials -
研究分野
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機能
Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens. -
配列類似性
Belongs to the arylamine N-acetyltransferase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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別名
- AAC1
- ARY1_HUMAN
- Arylamide acetylase 1
see all -
参照データベース
- Entrez Gene: 9 Human
- Entrez Gene: 116631 Rat
- Omim: 108345 Human
- SwissProt: P18440 Human
- SwissProt: P50297 Rat
- Unigene: 2 Human
- Unigene: 591847 Human
- Unigene: 37420 Rat
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab284533の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Enzyme activity assay |
Use at an assay dependent concentration.
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特記事項 |
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Enzyme activity assay
Use at an assay dependent concentration. |
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab284533 は論文での使用が確認できていません。