Anti-ARRDC3 抗体 (ab64817)
Key features and details
- Rabbit polyclonal to ARRDC3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ARRDC3 antibody -
製品の詳細
Rabbit polyclonal to ARRDC3 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
A synthetic peptide derived from the C-terminal of human ARRDC3.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab64817 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64817の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
The arrestins are a family of proteins that are important for regulating signal transduction within cells. Arrestins are part of a conserved two step mechanism for regulating the activity of G-protein coupled receptors (GPCRs). In response to a stimulus, GPCRs activate a heterotrimeric G protein. In order to turn off this response, or adapt to a constant stimulus, activated receptors need to be silenced. The first step is phosphorylation by a class of serine/threonine kinases called G protein coupled receptor kinases (GRKs). This phosphorylation specifically marks the activated receptor for arrestin binding. Once arrestin is bound to the receptor it is unable to signal further. Recent research continues to expand the known actions of arrestins, which can bind to other classes of receptors and can directly activate signaling pathways on their own. Different arrestins (visual arrestin (or Arrestin 1), beta-arrestin 1 (or Arrestin 2) and beta-arrestin 2 (or Arrestin 3) can reduce the activity of their target GPCRs in several different ways. -
細胞内局在
Cytoplasm. Note: Associated with plasma membrane, as well as with endodomes and lysosomes during endocytosis. -
参照データベース
- Entrez Gene: 57561 Human
- Entrez Gene: 105171 Mouse
- Entrez Gene: 309945 Rat
- Omim: 612464 Human
- SwissProt: Q96B67 Human
- SwissProt: Q7TPQ9 Mouse
- SwissProt: Q6TXF1 Rat
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別名
- ARRDC 3 antibody
- Arrestin domain containing 3 antibody
- Arrestin domain containing protein 3 antibody
see all
画像
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All lanes : Anti-ARRDC3 antibody (ab64817) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from COLO205 cells
Lane 3 : Extracts from COLO205 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa -
All lanes : Anti-ARRDC3 antibody (ab64817) at 1/500 dilution
Lane 1 : Rat brain lysates
Lane 2 : Mouse brain lysates
Lane 3 : Mouse brain lysates with immunizing peptide at 5 µg
Predicted band size: 46 kDa
Observed band size: 46 kDa -
IHC image of ab64817 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64817, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
ICC/IF image of ab64817 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64817, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab64817 は 9 報の論文で使用されています。
- Soung YH et al. The Role of Arrestin Domain-Containing 3 in Regulating Endocytic Recycling and Extracellular Vesicle Sorting of Integrin ß4 in Breast Cancer. Cancers (Basel) 10:N/A (2018). PubMed: 30545011
- Soung YH et al. Selective Inhibitors of Nuclear Export (SINE) compounds block proliferation and migration of triple negative breast cancer cells by restoring expression of ARRDC3. Oncotarget 8:52935-52947 (2017). PubMed: 28881784
- Tian X et al. The a-Arrestin ARRDC3 Regulates the Endosomal Residence Time and Intracellular Signaling of the ß2-Adrenergic Receptor. J Biol Chem 291:14510-25 (2016). Human . PubMed: 27226565
- Soung YH et al. Epigenetic silencing of ARRDC3 expression in basal-like breast cancer cells. Sci Rep 4:3846 (2014). WB ; Human . PubMed: 24457910
- Noh H et al. Prediction of miRNA-mRNA associations in Alzheimer's disease mice using network topology. BMC Genomics 15:644 (2014). WB, IP ; Mouse . PubMed: 25086961
- Puca L et al. ?-arrestin 1 (ARRDC1) and ß-arrestins cooperate to mediate Notch degradation in mammals. J Cell Sci 126:4457-68 (2013). WB . PubMed: 23886940
- Shea FF et al. Mammalian alpha arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with beta arrestins. PLoS One 7:e50557 (2012). ICC/IF ; Human . PubMed: 23236378
- Rauch S & Martin-Serrano J Multiple Interactions between the ESCRT Machinery and Arrestin-Related Proteins: Implications for PPXY-Dependent Budding. J Virol 85:3546-56 (2011). PubMed: 21191027
- Draheim KM et al. ARRDC3 suppresses breast cancer progression by negatively regulating integrin ß4. Oncogene 29:5032-47 (2010). IHC-Fr ; Human . PubMed: 20603614