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遺伝子ノックアウト細胞株 検証済

Anti-Arp5 抗体 (ab12099)

Reviews (2)Q&A (1)References (9)
Western blot - Anti-Arp5 antibody (ab12099)
  • Western blot - Anti-Arp5 antibody (ab12099)

Key features and details

  • Rabbit polyclonal to Arp5
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Saccharomyces cerevisiae
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Arp5 antibody

  • 製品の詳細

    Rabbit polyclonal to Arp5

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Saccharomyces cerevisiae

  • 免疫原

    Recombinant full length protein corresponding to Arp5.

  • ポジティブ･コントロール

    • S. cereviseae lysate

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab12099の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).

ターゲット情報

  • 関連性

    The budding yeast INO80 complex is a conserved ATP-dependent nucleosome remodeler containing actin-related proteins Arp5 and Arp8. Strains lacking INO80, ARP5, or ARP8 have defects in transcription. Ino80, Arp5, and Arp8 are recruited to an HO-induced DSB, where a phosphorylated form of H2A accumulates. Recruitment of Ino80 is compromised in cells lacking the H2A phosphoacceptor S129. INO80-mediated chromatin remodeling seems to occur directly at DSBs, where it appears to facilitate processing of the lesion.

  • 細胞内局在

    Nuclear

  • 参照データベース

    • 別名

      • Actin-like protein ARP5 antibody
      • ARP5_YEAST antibody
      • P53946 antibody

    画像

    • Western blot - Anti-Arp5 antibody (ab12099)
      Western blot - Anti-Arp5 antibody (ab12099)
      Anti-Arp5 antibody (ab12099) + S.cerevisiae lysate

      Secondary
      Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

      Predicted band size: 87 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?
      Additional bands at: 70 kDa (possible cross reactivity)

    • Western blot - Anti-Arp5 antibody (ab12099)
      Western blot - Anti-Arp5 antibody (ab12099)
      All lanes : Anti-Arp5 antibody (ab12099) at 0.36 µg/ml

      Lane 1 : S. cerevisiae whole cell lysate (wild type)
      Lane 2 : S. cerevisiae whole cell lysate (arp5 knockout)
      Lane 3 : S. cerevisiae whole cell lysate (arp8 knockout)
      Lane 4 : S. cerevisiae whole cell lysate (ino80 knockout)
      Lane 5 : S. cerevisiae whole cell lysate (anc1 knockout)
      Lane 6 : S. cerevisiae whole cell lysate (nhp10 knockout)
      Lane 7 : S. cerevisiae whole cell lysate (wild type, treated with MMS)
      Lane 8 : S. cerevisiae whole cell lysate (arp8 knockout, treated with MMS)

      Predicted band size: 87 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?



      Using ab12099, bands were present in all S. cerevisiae strains tested except for an arp5 knockout strain. This provides conclusive evidence that the ab12099 recognises Arp5.

    参考文献 (9)

    ab12099 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab12099 は 9 報の論文で使用されています。

    • Gowans GJ  et al. INO80 Chromatin Remodeling Coordinates Metabolic Homeostasis with Cell Division. Cell Rep 22:611-623 (2018). WB ; Saccharomyces cerevisiae . PubMed: 29346761
    • Xue Y  et al. Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals. Mol Cell 67:594-607.e4 (2017). PubMed: 28735899
    • Panday A & Grove A The high mobility group protein HMO1 functions as a linker histone in yeast. Epigenetics Chromatin 9:13 (2016). ChIP . PubMed: 27030801
    • Billant O  et al. The dominant-negative interplay between p53, p63 and p73: A family affair. Oncotarget 7:69549-69564 (2016). WB . PubMed: 27589690
    • Bennett G & Peterson CL SWI/SNF recruitment to a DNA double-strand break by the NuA4 and Gcn5 histone acetyltransferases. DNA Repair (Amst) 30:38-45 (2015). PubMed: 25869823
    • Panday A  et al. Yeast high mobility group protein HMO1 stabilizes chromatin and is evicted during repair of DNA double strand breaks. Nucleic Acids Res 43:5759-70 (2015). ChIP . PubMed: 25979266
    • Xue Y  et al. The Ino80 complex prevents invasion of euchromatin into silent chromatin. Genes Dev 29:350-5 (2015). ChIP-sequencing . PubMed: 25691465
    • Yao W  et al. Assembly of the Arp5 (Actin-related Protein) Subunit Involved in Distinct INO80 Chromatin Remodeling Activities. J Biol Chem 290:25700-9 (2015). PubMed: 26306040
    • Bennett G  et al. DNA repair choice defines a common pathway for recruitment of chromatin regulators. Nat Commun 4:2084 (2013). PubMed: 23811932

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    ChIP abreview for Anti-Arp5 antibody

     Good
    Abreviews
    abreview image
    Application
    ChIP
    Detection step
    Other
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (Whole cell lysate)
    Specification
    Whole cell lysate
    Negative control
    No Antibody sample
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 20 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 37% Formaldehyde
    Positive control
    Sample after 1 hour of DNA double strand break
    Read More

    Mr. Arvind Panday

    Verified customer

    投稿 Jul 09 2014

    Western blot abreview for Anti-Arp5 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    3.75e+006 cells
    Gel Running Conditions
    Reduced Denaturing (NUPAGE precasted Invitrogen 10% SDS-PAGE)
    Sample
    Schizosaccharomyces pombe Cell lysate - whole cell (whole cell extract)
    Specification
    whole cell extract
    Blocking step
    0.5% BSA + 5% non fat milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 17 2013

    Question

    it seems to me looking on your Western Blot that a-Arp5 antibody, cross interacts with Arp4 (band at 70 kDa). For my application it is essential that there is no cross-reactivity to Arp8 or Arp4. I have the similiar question concerning your Arp8 antibody. In the WB there are also additional bands (not the degradation). Do you know if on the WB of Arp5 of the knock-out studies the additional cross-reactive bands (not shown) vanish? Would it be possible to send me small aliqouts of those antibodies, that I can check the cross-reactivity? As like I said it is essential for our us to know this, before we order the antibodies.

    Read More
    Answer

    Thank you for contacting us. I have received the following information from the lab: Ab12099 (ARP5): Unfortunately, the full WB image showing the knock-out experiment is not on file, thus we are not able to determine if there were additional bands on the blot. We believe the testing may have been done by a collaborator a while ago, but currently do not have information to confirm that. We have not tested for cross reactivity with Arp4 or Arp8. The immunogen is the full length recombinant protein, thus we do not know where the epitope is located. The sequence homologies for the Arp5 yeast protein are as follows: Arp5-Arp4 = 21-30% (with lots of gaps) Arp5-Arp8 = very poor (30-53% for very short stretches, have lots of gaps) Thus, crossreactivity is quite unlikely. Usually a homology of 85% or higher is likely for cross reactivity, but not as low as 30%. Ab12098 (ARP8): We have no information on whether this antibody crossreacts with Arp4 and Arp5. The immunogen for this antibody is the full length recombinant protein. Again, we do not know the epitope region as it has not been mapped. The sequence homologies for the Arp5 yeast protein are as follows: Arp8-Arp4 = very poor (22-38% for short stretches, have lots of gaps) Arp8-Arp5 = very poor (30-53% for short stretches, have lots of gaps) Again, crossreactivity is quite unlikely as stated above. The expected MW of the unprocessed precursors for each yeast protein are Arp5 = 87 kDa Arp8 = 100 kDa Arp4 = 55 kDa Any other bands of different MW could be non-specific bands (but not Arp4,5 or 8). Unfortunately, we do not send out small aliquots as this is operationally not feasible for us. However, we do guarantee all our products for tested species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise. Should the antibodies not give you satisfactory results, we will be able to replace, credit or refund them. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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