Anti-Arp2 (phospho T237 + T238) 抗体 (ab119766)
Key features and details
- Rabbit polyclonal to Arp2 (phospho T237 + T238)
- Suitable for: WB, ICC
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Arp2 (phospho T237 + T238) antibody
Arp2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Arp2 (phospho T237 + T238) -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Chicken, Fish -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Rat PC12 cells differentiated with NGF. Human A431 and Jurkat cells treated with Calyculin A.
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特記事項
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab119766の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 32, 44 kDa (predicted molecular weight: 45 kDa).
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ICC |
1/100.
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特記事項 |
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WB
1/1000. Detects a band of approximately 32, 44 kDa (predicted molecular weight: 45 kDa). |
ICC
1/100. |
ターゲット情報
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機能
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. -
配列類似性
Belongs to the actin family. ARP2 subfamily. -
細胞内局在
Cytoplasm > cytoskeleton. Cell projection. - Information by UniProt
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参照データベース
- Entrez Gene: 396147 Chicken
- Entrez Gene: 10097 Human
- Entrez Gene: 66713 Mouse
- Entrez Gene: 289820 Rat
- Omim: 604221 Human
- SwissProt: P53488 Chicken
- SwissProt: P61160 Human
- SwissProt: P61161 Mouse
see all -
別名
- Actin like protein 2 antibody
- Actin related protein 2 antibody
- Actin-like protein 2 antibody
see all
画像
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All lanes : Anti-Arp2 (phospho T237 + T238) antibody (ab119766) at 1/1000 dilution
Lane 1 : A431 cells treated with Calyculin A
(100 nM) for 30 min.
Lane 2 : A431 cells treated with Calyculin A
(100 nM) for 30 min and treated with lambda phosphatase
Predicted band size: 45 kDa -
ab119766 at 1/100 dilution staining Arp2 (phospho T237 + T238) in rat PC12 cells differentiated with NGF (right).
Detection used an appropriate secondary antibody conjugated to Cy3.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab119766 は 3 報の論文で使用されています。
- Liu Y et al. Direct interaction between CEP85 and STIL mediates PLK4-driven directed cell migration. J Cell Sci 133:N/A (2020). PubMed: 32107292
- Miller HE et al. Actin polymerization in the endosomal pathway, but not on the Coxiella-containing vacuole, is essential for pathogen growth. PLoS Pathog 14:e1007005 (2018). PubMed: 29668757
- Li Z et al. Aging-Impaired Filamentous Actin Polymerization Signaling Reduces Alveolar Macrophage Phagocytosis of Bacteria. J Immunol 199:3176-3186 (2017). PubMed: 28947541