Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (pancreatic cells from Estrogen Receptor+ tumor)
Specification
pancreatic cells from Estrogen Receptor+ tumor
Fixative
Formaldehyde
Permeabilization
Yes - PBS + Triton 0.025%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Other product details
Dilution
1/200
Incubation time
4 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 488 Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 01 2010