製品の概要

  • 製品名

  • 製品の詳細

    Rabbit polyclonal to Aromatase
  • 由来種

    Rabbit
  • 特異性

    ab18995 recognises aromatase.
  • アプリケーション

    適用あり: ICC/IF, IHC-Fr, IHC-P, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Cow, Pig
  • 免疫原

    Synthetic peptide surrounding amino acid 385 of human Aromatase (Peptide available as ab51924.)

  • ポジティブ・コントロール

    • ICC/IF: HepG2 cells. IHC-P: Human normal placenta.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 19620711
IHC-P Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa). An additional 35 kDa band can also be detected in Jurkat cells (we are unsure of the identity).

ターゲット情報

  • 機能

    Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • 組織特異性

    Brain, placenta and gonads.
  • 関連疾患

    Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
    Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
  • 配列類似性

    Belongs to the cytochrome P450 family.
  • 細胞内局在

    Membrane.
  • Information by UniProt
  • 参照データベース

  • 別名

    • ARO antibody
    • ARO1 antibody
    • Aromatase antibody
    • CP19A_HUMAN antibody
    • CPV1 antibody
    • CYAR antibody
    • CYP19 antibody
    • Cyp19a1 antibody
    • CYPXIX antibody
    • Cytochrome P-450AROM antibody
    • Cytochrome P450 19A1 antibody
    • Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody
    • Cytochrome P450, subfamily XIX (aromatization of androgens) antibody
    • Estrogen synthase antibody
    • Estrogen synthetase antibody
    • Flavoprotein linked monooxygenase antibody
    • MGC104309 antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000162543 antibody
    • OTTHUMP00000198350 antibody
    • P 450AROM antibody
    see all

画像

  • Ab18995 staining Human normal placenta. Staining is localised to cell membrane.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab18995 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18995, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Aromatase antibody (ab18995) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa
    Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute

参考文献

This product has been referenced in:

  • Bogus K  et al. Chronic Antipsychotic Treatment Modulates Aromatase (CYP19A1) Expression in the Male Rat Brain. J Mol Neurosci 68:311-317 (2019). Read more (PubMed: 30968339) »
  • Berthon A  et al. High expression of adrenal P450 aromatase (CYP19A1) in association with ARMC5-primary bilateral macronodular adrenocortical hyperplasia. J Steroid Biochem Mol Biol 191:105316 (2019). Read more (PubMed: 31014964) »
See all 33 Publications for this product

レビューと Q&A

1-10 of 12 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Bos taurus Tissue sections (Ovary)
Permeabilization
Yes - Triton 0.3%
Specification
Ovary
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Oct 08 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Ovary)
Permeabilization
No
Specification
Ovary
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Ms. Natasja Costermans

Verified customer

投稿 Aug 09 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat Placenta)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Rat Placenta
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1.5%
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Mar 29 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - TritonX 100
Specification
Spinal cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Sep 29 2015

Answer



I have had a look at the western blot from the other distributor and it does indeed show two bands at similar molecular weights to the ones shown on our datasheet. We have also looked at similar antibodies from other companies and their antibodies also show these two characteristic bands at ˜34kDA and ˜58 kDa.

Some of these other suppliers make reference to the lower band possibly being the cleavage product of Aromatase however, we have not yet been able to find any online data regarding a potential cleavage product and therefore there is no data to support this hypothesis.

Since our last correspondence, we have also found http://humrep.oxfordjournals.org/content/24/9/2133.full which indicates that the lower band is not specific (by sequencing).

Hence, at present, we are still unsure as to the identity of this lower band.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pancreatic tumor)
Specification
pancreatic tumor
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Oct 08 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreatic tumor)
Specification
pancreatic tumor
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Oct 05 2012

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (brain)
Loading amount
15 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Apr 28 2011

Application
Western blot
Sample
Rat Tissue lysate - whole (Spinal Cord membrane)
Loading amount
15 µg
Specification
Spinal Cord membrane
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Vijaya Murugaiyan

Verified customer

投稿 Mar 24 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (pancreatic cells from Estrogen Receptor+ tumor)
Specification
pancreatic cells from Estrogen Receptor+ tumor
Fixative
Formaldehyde
Permeabilization
Yes - PBS + Triton 0.025%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

投稿 Oct 01 2010

1-10 of 12 Abreviews or Q&A

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