Armenian Hamster IgG monoclonal [HTK888] - Isotype Control (ab18479)

Q&A (5)References (2)

製品の概要

  • 製品名

    Armenian Hamster IgG monoclonal [HTK888] - Isotype Control
    Hamster アイソタイプ・コントロール 製品一覧

  • アプリケーション

    適用あり: IHC-P, IHC-Fr, IP, WB, Flow Cytmore details

  • 免疫原

    Trinitrophenol and KLH

  • 特記事項

    Isotype control has been screened on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    HTK888

  • アイソタイプ

    IgG

  • 研究分野

  • 別名

    • Hamster Isotype Control

アプリケーション

Our Abpromise guarantee covers the use of ab18479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
IHC-Fr
IP
WB
Flow Cyt
  • 追加情報
    Flow Cyt: Use at an assay dependent dilution for surface or intracellular staining.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: n/a kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 参考文献 (2)

    ab18479 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab18479 は 2 報の論文で使用されています。

    • Matsuo K  et al. Innervation of the tibial epiphysis through the intercondylar foramen. Bone 120:297-304 (2018). PubMed: 30439572
    • Hoffhines AJ  et al. Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo. J Biol Chem 284:3096-105 (2009). PubMed: 19047058

    レビューと Q&A

    Q&A
    質問を送る

    1-5 of 5 Q&A

    Question

    We have purchased some antibodies against rat platelets:

    Primary:

    ab108949 - mouse monoclonal anti CD63, FITC labeled
    (ab106163 as isotype control)

    ab54427 - mouse monoclonal anti CD62P
    (ab91353 as the isotype control, as a secondary antibody)

    ab95780 anti CD42d armenian hamster antibody
    (ab18479 as an isotype control, for which we are using APC-conjugated Goat Anti-Hamster IgG from Leinco).

    We are ibodies.doing flow cytometry experiments on fixed whole blood, and fixed platelet-rich plasma. The problem is that the samples with the isotype control show the same fluorescence as the samples with the primary ant We have tried a number of different fixing and staining protocols, with and without washing, but until now had no success. What I am wondering about is, how reliable are these primary antibodies for the use with rat platelets?

    Some help would be greatly appreciated.

    Read More
    Answer

    Thank you for your reply.

    I am sorry that you have been having issues with some of our antibodies in Flow Cytometry. Under our Abpromise we guarantee that ab108949, ab54427 & ab95780 should work in flow cytometry on rat samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

    To be able to help you further in this matter, would you be able to send me a copy of the protocol that you have been using, along with any modifications that you have tried, to see if there is anything I can suggest.

    I look forward to your reply and helping resolve this matter.

    Read More

    Question

    Yes, it is correct. ************does the purchasing in this group usually. I am a visiting professor here.

    Feel free to contact him to double-check, he is the one who gave me the customer ID.

    Read More
    Answer

    Thank you for your reply.

    I have sent the quote for the products requested to the email address we have on file for **********.

    When you are ready to convert the quote to an order, please contact our customer service team on 1-888-772-2226 or email to mailto:us.orders@abcam.com.

    If there is anything else I can help you with, please let me know.

    Read More

    Question

    Concerning the quote, the customer number is**********

    Read More
    Answer

    Thank you for your reply.

    Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just wanted to make sure that the quote went to the correct person.

    Thank you for your patience, and I look forward to your reply.

    Read More

    Question

    I selected the following antibodies from the ABcam catalog:

    Primary:

    Anti-CD63 antibody [AD1] (FITC)

    ab108949

    $337.00



    Anti-CD62P antibody [Psel.KO.2.7]

    ab54427

    $337.00



    Anti-CD42d antibody [1C2]

    ab95780

    $337.00



    Secondary:


    Goat anti-Mouse IgG H&L (Cy5.5 ®)

    ab6947

    $185.00



    Goat anti-Mouse IgG1 heavy chain (FITC)

    ab97239

    $165.00



    Goat polyclonal to Armenian Hamster (TR)

    ab5743

    $165.00



    Isotype controls:


    MOUSE IgG1 antibody [MOPC-21] (FITC)

    ab106163

    $325.00



    Mouse IgG1 [ICIGG1] - Isotype Control

    ab91353

    $315.00



    Armenian Hamster IgG monoclonal [HTK888] - Isotype control

    ab18479

    $325.00



    Two questions:

    For the secondary antibody, will the first one (ab6947) work against ab54427, or do we need to buy the second one (ab97239)? One is against IgG, the other one is against IgG1. ab54427 is IgG1.

    If you could double-check the selection for compatibility (e.g., correct isotype controls) and then ask the sales department to offer us a quote, that would be great.

    Read More
    Answer

    Thank you for your reply.

    The antibodies you selected look good to me. To generate a quote we do need to have a shipping address and in your last email, you mentioned that you were at the University of Toronto at the moment. Could you please send me your current email address and I will generate the quote.

    I look forward to your reply.

    Read More

    Question

    I need to use a control antibody for a functional study with your Integrin beta 3 antibody(Cat. ab36436). Could you please let me know which form of Ig is it ? for example: Hamster IgG (IgG1 or G2)?

    Read More
    Answer

    Thank you for contacting us with your question. I contacted the production lab to enquire about the isotype of ab36436, and unfortunately they said that the antibody has not been characterized to distinguish if it is IgG1 or IgG2.   We do have hamster IgG isotype controls that you may use as control antibodies. I would recommend ab18479, which is an Armenian Hamster IgG isotype control. This antibody has been tested on a variety of mouse, rat, and human tissues. I hope this information is helpful, but please let me know if you have further questions.  

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com