Anti-ARIH1 抗体 (ab3891)
Key features and details
- Goat polyclonal to ARIH1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ARIH1 antibody
ARIH1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ARIH1 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
HEGYEKDLWEYIED
, corresponding to C terminal amino acids 544-557 of Human ARIH1. -
ポジティブ･コントロール
- U937 lysate .
-
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab3891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Recommended for use at 0.2-1.0µg/ml.
Not tested in other applications.
Optimal working dilutions / concentrations should be determined by the end user.
ターゲット情報
-
機能
Might act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the RBR family. Ariadne subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers. -
細胞内局在
Cytoplasm. Mainly cytoplasmic. - Information by UniProt
-
参照データベース
- Entrez Gene: 25820 Human
- Omim: 605624 Human
- SwissProt: Q9Y4X5 Human
- Unigene: 268787 Human
-
別名
- ARI 1 antibody
- ARI antibody
- ARI-1 antibody
see all
画像
-
Western blot - Anti-ARIH1 antibody (ab3891)This image is courtesy of an Abreview submitted by T LawsonAll lanes : Anti-ARIH1 antibody (ab3891) at 1/1500 dilution
Lanes 1-2 : NIH3T3 whole cell lysate at 30 µg
Lane 3 : Partially purified NIH3T3 protein mixture at 10 µg
Secondary
All lanes : Alkaline Phosphatase-conjugated anti-goat IgG polyclonal at 1/15000 dilution
Performed under reducing conditions.
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)
Exposure time: 15 minutesLanes 1 and 2 - 12% SDS-PAGE.
Lane 3 - 4-15% SDS-PAGE.
Lanes 1 and 3 - developed with colorimetric substrate.
Lane 2 - developed with ECF+.
-
Western blot - Anti-ARIH1 antibody (ab3891)ab3891 staining (0.5µ g/ml) of U937 lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab3891 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
データシートおよび資料
参考文献 (5)
ab3891 は 5 報の論文で使用されています。
- Tan KL et al. Ari-1 Regulates Myonuclear Organization Together with Parkin and Is Associated with Aortic Aneurysms. Dev Cell 45:226-244.e8 (2018). PubMed: 29689197
- Polajnar M et al. Expanding the host cell ubiquitylation machinery targeting cytosolic Salmonella. EMBO Rep 18:1572-1585 (2017). PubMed: 28784601
- Scott DC et al. Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation. Cell 166:1198-1214.e24 (2016). PubMed: 27565346
- Wong ES et al. Relative sensitivity of parkin and other cysteine-containing enzymes to stress-induced solubility alterations. J Biol Chem 282:12310-8 (2007). PubMed: 17329252
- LaVoie MJ et al. The effects of oxidative stress on parkin and other E3 ligases. J Neurochem 103:2354-68 (2007). PubMed: 17883392
