Anti-ARIH1 抗体 (ab3891)
Key features and details
- Goat polyclonal to ARIH1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ARIH1 antibody
ARIH1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ARIH1 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
HEGYEKDLWEYIED
, corresponding to C terminal amino acids 544-557 of Human ARIH1. -
ポジティブ･コントロール
- U937 lysate .
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab3891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
追加情報
Western Blot: Approx 65 kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735].
Recommended for use at 0.2-1.0µg/ml.
Not tested in other applications.
Optimal working dilutions / concentrations should be determined by the end user.
Recommended for use at 0.2-1.0µg/ml.
Not tested in other applications.
Optimal working dilutions / concentrations should be determined by the end user.
ターゲット情報
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機能
Might act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the RBR family. Ariadne subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers. -
細胞内局在
Cytoplasm. Mainly cytoplasmic. - Information by UniProt
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参照データベース
- Entrez Gene: 25820 Human
- Omim: 605624 Human
- SwissProt: Q9Y4X5 Human
- Unigene: 268787 Human
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別名
- ARI 1 antibody
- ARI antibody
- ARI-1 antibody
see all
画像
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Western blot - Anti-ARIH1 antibody (ab3891)This image is courtesy of an Abreview submitted by T LawsonAll lanes : Anti-ARIH1 antibody (ab3891) at 1/1500 dilution
Lanes 1-2 : NIH3T3 whole cell lysate at 30 µg
Lane 3 : Partially purified NIH3T3 protein mixture at 10 µg
Secondary
All lanes : Alkaline Phosphatase-conjugated anti-goat IgG polyclonal at 1/15000 dilution
Performed under reducing conditions.
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)
Exposure time: 15 minutesLanes 1 and 2 - 12% SDS-PAGE.
Lane 3 - 4-15% SDS-PAGE.
Lanes 1 and 3 - developed with colorimetric substrate.
Lane 2 - developed with ECF+.
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Western blot - Anti-ARIH1 antibody (ab3891)ab3891 staining (0.5µ g/ml) of U937 lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab3891 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
データシートおよび資料
参考文献 (5)
ab3891 は 5 報の論文で使用されています。
- Tan KL et al. Ari-1 Regulates Myonuclear Organization Together with Parkin and Is Associated with Aortic Aneurysms. Dev Cell 45:226-244.e8 (2018). PubMed: 29689197
- Polajnar M et al. Expanding the host cell ubiquitylation machinery targeting cytosolic Salmonella. EMBO Rep 18:1572-1585 (2017). PubMed: 28784601
- Scott DC et al. Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation. Cell 166:1198-1214.e24 (2016). PubMed: 27565346
- Wong ES et al. Relative sensitivity of parkin and other cysteine-containing enzymes to stress-induced solubility alterations. J Biol Chem 282:12310-8 (2007). PubMed: 17329252
- LaVoie MJ et al. The effects of oxidative stress on parkin and other E3 ligases. J Neurochem 103:2354-68 (2007). PubMed: 17883392
