Anti-ARID1A 抗体 [EPR13501-73] (ab182561)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13501-73] to ARID1A
- Suitable for: ChIC/CUT&RUN-seq, WB, Flow Cyt (Intra), ICC/IF, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-ARID1A antibody [EPR13501-73]
ARID1A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13501-73] to ARID1A -
由来種
Rabbit -
アプリケーション
適用あり: ChIC/CUT&RUN-seq, WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Wild-type HAP1 treated with 10uM MG-132 for 6 hours whole cell lysate. Flow-Cyt (intra): SH-SY5Y cells. IHC-P: Human kidney and Human adenocarcinoma of endometrium without ARID1A mutation. ICC/IF: SH-SY5Y cells. ChIC/CUT&RUN-Seq: HCT116 cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13501-73 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab182561の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ChIC/CUT&RUN-seq |
Use at an assay dependent concentration.
5µg |
|
WB |
1/1000. Predicted molecular weight: 242 kDa.
|
|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
ICC/IF | (1) |
1/500.
|
IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
特記事項 |
---|
ChIC/CUT&RUN-seq
Use at an assay dependent concentration. 5µg |
WB
1/1000. Predicted molecular weight: 242 kDa. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
1/500. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Binds DNA non-specifically. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. -
組織特異性
Highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and PBL, and at a much lower level in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. -
配列類似性
Contains 1 ARID domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 8289 Human
- Entrez Gene: 93760 Mouse
- Entrez Gene: 297867 Rat
- Omim: 603024 Human
- SwissProt: O14497 Human
- SwissProt: A2BH40 Mouse
- Unigene: 468972 Human
- Unigene: 22478 Mouse
-
別名
- actin-dependent regulator of chromatin subfamily F member 1 antibody
- ARI1A_HUMAN antibody
- ARID domain containing protein 1A antibody
see all
画像
-
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HCT116 cells and 5µg of ab182561 [EPR13501-73]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
Additional screenshots of mapped reads can be downloaded here.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
-
All lanes : Anti-ARID1A antibody [EPR13501-73] (ab182561) at 1/1000 dilution
Lane 1 : ARID1 knockout HAP1 (Human chronic myelogenous leukemia near-haploid cell line) treated with 10uM MG-132 for 6 hours whole cell lysate
Lane 2 : Wild-type HAP1 (Human chronic myelogenous leukemia-haploid cell line) treated with 10uM MG-132 for 6 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 242 kDa
Observed band size: 130-270 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and diluting buffer: 5% NFDM/TBST
ARID1A has many mutations which typically generate truncated proteins that are highly prone to degradation. (PMID: 21614196, PMID: 29486633, PMID: 34429326).
-
Immunohistochemical analysis of paraffin-embedded Human kidney tissue (left image) labeling ARID1A using ab182561 at 1/1000 dilution. Right image: human clear cell carcinoma of kidney with ARID1A mutation. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
Immunofluorescent analysis of SH-SY5Y cells labeling ARID1A with ab182561 at 1/500 and Goat anti rabbit IgG(Alexa Fluor®555) at 1/200. Image at the right stained with DAPI.
-
Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling ARID1A with purified ab92512 at 1/500 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
-
Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium without ARID1A mutation (left image) labeling ARID1A using ab182561 at 1/1000 dilution. Right image: human adenocarcinoma of endometrium with ARID1A mutation. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (7)
ab182561 は 7 報の論文で使用されています。
- Munakata S & Yamamoto T Application of immunocytochemical and molecular analysis of six genes in liquid-based endometrial cytology. Diagn Cytopathol 50:8-17 (2022). PubMed: 34783431
- Megino-Luque C et al. ARID1A-deficient cells require HDAC6 for progression of endometrial carcinoma. Mol Oncol 16:2235-2259 (2022). PubMed: 35167193
- Wedge ME et al. Virally programmed extracellular vesicles sensitize cancer cells to oncolytic virus and small molecule therapy. Nat Commun 13:1898 (2022). PubMed: 35393414
- Du J et al. Arid1a-Plagl1-Hh signaling is indispensable for differentiation-associated cell cycle arrest of tooth root progenitors. Cell Rep 35:108964 (2021). PubMed: 33826897
- Kishida Y et al. Associations Between Loss of ARID1A Expression and Clinicopathologic and Genetic Variables in T1 Early Colorectal Cancer. Am J Clin Pathol 152:463-470 (2019). PubMed: 31263894
- Jing J et al. Antagonistic interaction between Ezh2 and Arid1a coordinates root patterning and development via Cdkn2a in mouse molars. Elife 8:N/A (2019). PubMed: 31259687
- Huang KK et al. Genomic and Epigenomic Profiling of High-Risk Intestinal Metaplasia Reveals Molecular Determinants of Progression to Gastric Cancer. Cancer Cell 33:137-150.e5 (2018). PubMed: 29290541