Anti-Argonaute-2 抗体 - ChIP Grade (ab32381)


  • 製品名
    Anti-Argonaute-2 antibody - ChIP Grade
    Argonaute-2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Argonaute-2 - ChIP Grade
  • 由来種
  • アプリケーション
    適用あり: IP, IHC-P, ChIP, RIP, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Cow非交差種: Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to Argonaute-2 aa 350-450 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab32380)

  • ポジティブ・コントロール
    • ab32381 gives a positive result in Western Blot in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line), Jurkat (Human T cell lymphoblast-like cell line), HeLa (Human epithelial carcinoma cell line) Nuclear Lysate



Our Abpromise guarantee covers the use of ab32381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration. PubMed: 24280866
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP Use at an assay dependent concentration. PubMed: 19696410
RIP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 97 kDa).
ICC/IF 1/200.


  • 機能
    Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
  • 配列類似性
    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • ドメイン
    The Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However while RNase H utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of Asp-Asp-His (DDH).
  • 翻訳後修飾
    Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity.
  • 細胞内局在
    Cytoplasm > P-body. Nucleus. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ago 2 antibody
    • AGO2_HUMAN antibody
    • Argonaute 2 antibody
    • argonaute 2, RISC catalytic component antibody
    • Argonaute RISC catalytic component 2 antibody
    • Argonaute2 antibody
    • CTA-204B4.6 antibody
    • dAgo2 antibody
    • eIF 2C 2 antibody
    • eIF-2C 2 antibody
    • eIF2C 2 antibody
    • Eif2c2 antibody
    • Eukaryotic translation initiation factor 2C 2 antibody
    • Eukaryotic translation initiation factor 2C subunit 2 antibody
    • hAgo2 antibody
    • MGC3183 antibody
    • PAZ Piwi domain protein antibody
    • PPD antibody
    • Protein argonaute-2 antibody
    • Protein slicer antibody
    • Q10 antibody
    • Slicer protein antibody
    see all


  • All lanes : Anti-Argonaute-2 antibody - ChIP Grade (ab32381) at 2 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 87 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.

    The identification of the 55 kDa band is unclear but this band has also been observed in Hela lysates in the Western Blot of ab5072, targeting Drosophila Ago2 / eIF2C2.

  • IHC image of Ago2/eIF2C2 staining in human breast cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32381, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab32381 (1/200) staining Ago2/ eIF2C2 in assynchrnous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% TritonX100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

  • RIP analysis of 368T1 murine lung cancer cells using ab32381 to bind Ago2 / eIF2C2.

    Cells were transfected with an empty vector (empty), a vector expressing wild-type Hmga2 (wt), a vector expressing a let-7 site mutated Hmga2 (m7), a vector expressing wild-type Hmga2 without a start codon (ATG wt), or a vector expressing a let-7 site mutated Hmga2 without a start codon (ATG m7). RIP was performed with 5 µg Ago2 / eIF2C2 or IgG antibody per 107 cells. Binding was detected using qRT-PCR.

    See Abreview


This product has been referenced in:
  • Krause M  et al. Exosomes as secondary inductive signals involved in kidney organogenesis. J Extracell Vesicles 7:1422675 (2018). Read more (PubMed: 29410779) »
  • Antoniou A  et al. The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development. EMBO Rep 19:N/A (2018). IF ; Rat . Read more (PubMed: 29263199) »

See all 45 Publications for this product

レビューと Q&A

We do not have any cross-reactivity data for this product with other argonaute proteins. However, the sequence homology analysis suggests it is unlikely this antibody would cross-react with Ago1, Ago3 or Ago4. Human Ago1 : 69% homology Human Ago3 : ...

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Thank you for contacting us.No, we have not stained mouse primary cells for confocal microscopy, although mouse tissue has been tested as well as human cell cultures.

I hope this information is helpful to you. Please do not hesitate to co...

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Thank you for your reply.

I have sent you 2 new vials of ab32381, since the original 2 we sent did not work in western blotting for you. Your new order number is********and you should hopefully have them by Friday.

If there is anyth...

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Thank you for contacting Abcam earlier today.

I just noticed on that we shipped 2 vials of ab32381 on the order information that you provided. I just wanted to check if both of these vials were for your lab and therefore need both vials repla...

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Thank you for contacting Abcam.

The alignment with mouse is also excellent with a 98% homology. Staining of mouse tissue using mouse antibody is a complicated process as high levels of background are often observed. It is notoriously difficult...

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Thank you very much for your interest in ab32381 and ab57113.

Bothantibodie arenot tested and guaranteed for rat and CLIP. It is tested for mouse samples but also not for CLIP. We do not know yet if it will work. Since rat is predicted to wor...

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Thank you for contacting Abcam.

The alignment with rat is also excellent with a 95% homology. Again this is not covered under our abpromise but is eligible for the Abreview discount program

Please let me know if you have any questions.

Thank you for contacting us.

We do offer another product, ab57113, which has been tested in ChIP and has a better signal. This product has not yet been tested in mouse however. I ran an alignment between the human Ago2 sequence used for creat...

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Thank you for contacting us. The alignment results I obtained are: SpAgo1 and SpAgo2 69% identity PmAgo1/2 75% identity. There are some gaps, rather than contiguous conserved sequence, and I am reluctant to recommend this antibody for detecti...

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At this time we have no data regarding the use of ab32381 in CLIP. So we would not be able to offer the Abpromise for the use of this antibody in CLIP. I would happy to set up a discount code for your customer to test ab32381 in CLIP.