Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Fruit fly (Drosophila melanogaster) Cell (OSS cells)
Specification
OSS cells
Permeabilization
Yes - Tween 0,2%
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Dilution
1/5000
Additional data
Additional Notes
The Mab 414 is the antibody Anti-Nuclear Pore Complex Proteins antibody [Mab414] - ChIP Grade (ab24609) . I use Mab 414 (red) as a nuclear membrane marker and I obtain a very good labeling as expected. There is no Hoescht or DAPI stainning but I checked with other staining that Mab414 has a nuclear membrane localisation.
Green Ago2: In wild type cells OSS Ago 2 have a nuclear localisation.We observed a nuclear spot.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Nathalie Gred Gueguen
Verified customer
投稿 Sep 15 2014