Anti-ARF1 抗体 [ARFS 3F1] (ab11038)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)](https://www.abcam.co.jp/ps/products/11/ab11038/Images/ab11038-18717-anti-arf1-antibody-arfs-3f1-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)")
Key features and details
- Mouse monoclonal [ARFS 3F1] to ARF1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-ARF1 antibody [ARFS 3F1]
ARF1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [ARFS 3F1] to ARF1 -
由来種
Mouse -
特異性
This antibody is specific to class I ARF. -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
SNQLRNQ
, corresponding to C terminal amino acids 174-180 of Human ARF1. -
ポジティブ･コントロール
- BALB/3T3 whole cell lysate (ab7901) can be used as a positive control in WB.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
ARFS 3F1 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11038の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. -
配列類似性
Belongs to the small GTPase superfamily. Arf family. -
細胞内局在
Golgi apparatus. Cytoplasm > perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 375 Human
- Omim: 103180 Human
- SwissProt: P84077 Human
- Unigene: 286221 Human
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別名
- ADP Ribosylation Factor 1 antibody
- ADP-ribosylation factor 1 antibody
- ARF 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)IHC image of ab11038 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11038, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab11038 は 8 報の論文で使用されています。
- Xu J & Zhang J LncRNA TP73-AS1 is a novel regulator in cervical cancer via miR-329-3p/ARF1 axis. J Cell Biochem 121:344-352 (2020). PubMed: 31232491
- Tan JZA et al. Distinct anterograde trafficking pathways of BACE1 and amyloid precursor protein from the TGN and the regulation of amyloid-ß production. Mol Biol Cell 31:27-44 (2020). PubMed: 31746668
- Pye DS et al. Chemokine unresponsiveness of chronic lymphocytic leukemia cells results from impaired endosomal recycling of Rap1 and is associated with a distinctive type of immunological anergy. J Immunol 191:1496-504 (2013). PubMed: 23804711
- Lim J et al. The CNK1 scaffold binds cytohesins and promotes insulin pathway signaling. Genes Dev 24:1496-506 (2010). WB ; Human . PubMed: 20634316
- Tang D et al. Molecular mechanism of mitotic Golgi disassembly and reassembly revealed by a defined reconstitution assay. J Biol Chem 283:6085-94 (2008). WB . PubMed: 18156178
- Havrylov S et al. Adaptor protein Ruk/CIN85 is associated with a subset of COPI-coated membranes of the Golgi complex. Traffic 9:798-812 (2008). PubMed: 18266907
- Cotton M et al. Endogenous ARF6 interacts with Rac1 upon angiotensin II stimulation to regulate membrane ruffling and cell migration. Mol Biol Cell 18:501-11 (2007). PubMed: 17122362
- Yano H et al. Fbx8 Makes Arf6 Refractory to Function via Ubiquitination. Mol Biol Cell : (2007). WB ; Human . PubMed: 18094045
