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リコンビナントRabMAb

Anti-ARF1 + ARF3 抗体 [EPR443] (ab108347)

Submit a review Submit a question References (6)
Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
  • Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
  • Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
  • Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR443] to ARF1 + ARF3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647

製品の概要

  • 製品名

    Anti-ARF1 + ARF3 antibody [EPR443]
    ARF1+ARF3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR443] to ARF1 + ARF3

  • 由来種

    Rabbit

  • 特異性

    The immunogen used to raise this antibody shares 100% homology with human ARF3, 96% homology with ARF4 and ARF5, and 88% homology with ARF6. Cross-reactivity with the latter three proteins have not been confirmed experimentally.

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human, Recombinant fragment
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human fetal kidney, Human fetal brain and MCF7 lysates; Human breast ductal carcinoma, and Human colonic adenocarcinoma tissues This antibody gave a positive result when used in the following methanol fixed cell lines: CaCo2

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108347の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

画像

  • Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    All lanes : Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/50000 dilution

    Lane 1 : Full length human ARF-1 recombinant protein
    Lane 2 : Full length human ARF-3 recombinant protein

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM /TBST

    Human ARF-1 recombinant protein, (Cat#:ab123538 )
    Human ARF-3 recombinant protein, (Cat#:ab113581 )

  • Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

    ICC/IF image of ab108347 stained CaCo2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108347 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    All lanes : Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : MCF7 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

    Immunohistochemical analysis of ARF1 in paraffin embedded Human breast ductal carcinoma tissue, using ab108347 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

    Immunohistochemical analysis of ARF1 in paraffin embedded Human colonic adenocarcinoma tissue, using ab108347 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

参考文献 (6)

ab108347 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108347 は 6 報の論文で使用されています。

  • Liu Q  et al. Histone demethylase PHF8 drives neuroendocrine prostate cancer progression by epigenetically upregulating FOXA2. J Pathol 253:106-118 (2021). PubMed: 33009820
  • Zhu T  et al. TRPM2 channel regulates cytokines production in astrocytes and aggravates brain disorder during lipopolysaccharide-induced endotoxin sepsis. Int Immunopharmacol 75:105836 (2019). PubMed: 31450153
  • Rafiq NB  et al. Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. J Cell Biol 216:181-197 (2017). WB . PubMed: 28007915
  • Eiseler T  et al. Protein Kinase D2 Assembles a Multiprotein Complex at the Trans-Golgi Network to Regulate Matrix Metalloproteinase Secretion. J Biol Chem 291:462-77 (2016). PubMed: 26507660
  • Schultz ML  et al. CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and actin-dependent events. PLoS One 9:e96647 (2014). WB ; Mouse . PubMed: 24792215
  • Jung JJ  et al. Secretion of soluble vascular endothelial growth factor receptor 1 (sVEGFR1/sFlt1) requires Arf1, Arf6, and Rab11 GTPases. PLoS One 7:e44572 (2012). PubMed: 22962618

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