Anti-Aquaporin 4 抗体 [EPR7040] - BSA and Azide free (ab248213)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7040] to Aquaporin 4 - BSA and Azide free
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Aquaporin 4 antibody [EPR7040] - BSA and Azide free
Aquaporin 4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7040] to Aquaporin 4 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IHC-Fr, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Mouse stomach, Mouse kidney, Rat stomach, Rat kidney and Mouse brain, Human stomach lysates (unboiled); IHC-P: Human cerebrum, Human stomach, Mouse cerebrum, Mouse cerebellum, Mouse stomach, Mouse kidney and Rat cerebrum, Rat stomach tissues. IHC-Fr: Rat stomach, Mouse stomach tissues.
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特記事項
ab248213 is the carrier-free version of ab128906.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 2.60 x 10 -12 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7040 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248213の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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IHC-Fr |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa. |
ターゲット情報
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機能
Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. -
組織特異性
Brain - muscle >> heart, kidney, lung, and trachea. -
配列類似性
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
ドメイン
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
翻訳後修飾
Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 361 Human
- Entrez Gene: 11829 Mouse
- Entrez Gene: 25293 Rat
- Omim: 600308 Human
- SwissProt: P55087 Human
- SwissProt: P55088 Mouse
- SwissProt: P47863 Rat
- Unigene: 315369 Human
see all -
別名
- AQP 4 antibody
- AQP-4 antibody
- AQP4 antibody
see all
画像
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All lanes : Anti-Aquaporin 4 antibody [EPR7040] (ab128906) at 1/1000 dilution
Lane 1 : Mouse brain lysate boiled
Lane 2 : Mouse brain lysate unboiled
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 100,28 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsThis data was developed using ab128906, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
'Unboil method' will optimize WB result.
We recommend not to boil the samples after lysis to get desired WB results.The molecular weight is consistent with what has been described in the literature. Bands observed at 100kDa relate to a tetramer of AQP4 (PMID:18334967, 11742978, 17897643).
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All lanes : Anti-Aquaporin 4 antibody [EPR7040] (ab128906) at 1/1000 dilution
Lane 1 : Mouse kidney lysate unboiled
Lane 2 : Mouse brain lysate unboiled
Lane 3 : Rat stomach lysate unboiled
Lane 4 : Rat kidney lysate unboiled
Lane 5 : Human stomach lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 100,28 kDa why is the actual band size different from the predicted?This data was developed using ab128906, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST. '
Unboil method' will optimize WB result.
Lane1 & Lane2: 20 seconds. Lane3 & Lane4 & Lane5: 120 seconds.
The molecular weight is consistent with what has been described in the literature. Bands observed at 100kDa relate to a tetramer of AQP4 (PMID:18334967, 11742978, 17897643).
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Anti-Aquaporin 4 antibody [EPR7040] (ab128906) at 1/1000 dilution + Human stomach lysate unboiled at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 28,30 kDa why is the actual band size different from the predicted?
Exposure time: 120 secondsThis data was developed using ab128906, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
'Unboil method' will optimize WB result.
The molecular weight is consistent with what has been described in the literature. (PMID: 22590566, 17897643).
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This data was developed using ab128906, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse stomach (fresh) tissue labeling Aquaporin 4 with ab128906 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution (Green). Positive staining on mouse stomach. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 µg/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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This data was developed using ab128906, the same antibody clone in a different buffer formµlation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat stomach (fresh) tissue labeling Aquaporin 4 with ab128906 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution (Green). Positive staining on rat stomach. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 µg/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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This data was developed using ab128906, the same antibody clone in a different buffer formµlation.
Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat stomach.The section was incubated with ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
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This data was developed using ab128906, the same antibody clone in a different buffer formµlation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat cerebrum.The section was incubated with ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
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This data was developed using ab128906, the same antibody clone in a different buffer formµlation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse kidney.The section was incubated with ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
-
This data was developed using ab128906, the same antibody clone in a different buffer formµlation.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse stomach.The section was incubated with ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
-
This data was developed using ab128906, the same antibody clone in a different buffer formµlation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse cerebellum.The section was incubated with ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
-
This data was developed using ab128906, the same antibody clone in a different buffer formµlation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse cerebrum.The section was incubated with ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
-
This data was developed using ab128906, the same antibody clone in a different buffer formµlation.
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human stomach.The section was incubated with ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
-
This data was developed using ab128906, the same antibody clone in a different buffer formµlation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human cerebrum.The section was incubated with ab128906 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
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This data was developed using ab128906, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248213 は論文での使用が確認できていません。