Anti-Aquaporin 4 抗体 [4/18] (ab9512)

Thank you for this information. It is unusual for one to see a band and then subsequently see nothing, which is why I thought you may have been stripping and re-probing. You may improve your result by increasing the concentration of the primary antibody further. Generally the actin band will always be a lot stronger since it is a highly expressed loading control. We can send you a free of charge replacement of the antibody if you wish to try a new lot. Please let me know if this is satisfactory.

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab9512 Anti-Aquaporin 4 antibody [4/18]. I would also appreciate if you can confirm some further details:


1. As this is a multipass membrane protein, which tent to aggregate in the loading buffer when boiled at high temperature due to their hydrophobic nature, I would like to recommend lowering the boiling temperature down to 60C, but keepingthe boiling time of10 min. In addition, use DTT instead of beta-mercaptoethanol as this kind of aggregation is favoured by b- ME.

2. Please be aware that there are two isoforms of this protein, one is 29 and one is 35 kDa.

3.For a better saturation of the membrane, I would recommend a blocking buffer containing 3% BSA for 2 hours at room temperature. BSA can sometimes give better results than milk.Please use the same agent during antibody incubation.

3. In order to increase the specific binding, I would like to recommend decreasing the antibodies concentration down 1:1500.


Should the suggestions not improve the results, please do let me know.

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I hope this information is helpful, and I thank you for your cooperation.

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