Anti-Aquaporin 2 抗体 [EPR21080] (ab199975)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21080] to Aquaporin 2
- Suitable for: WB, IHC-P, IHC-Fr, IP, mIHC
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Aquaporin 2 antibody [EPR21080]
Aquaporin 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR21080] to Aquaporin 2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IHC-Fr, IP, mIHCmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human fetal kidney lysate; Mouse kidney lysate. IHC-P: Human, mouse and rat kidney tissues. IHC-Fr: Mouse and rat kidney tissues. IP: Aquaporin 2 IP in mouse kidney lysate. mIHC: Human kidney tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab199975の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500. Predicted molecular weight: 28 kDa.
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IHC-P | (1) |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
1/3000.
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IP |
1/30.
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mIHC |
1/4000.
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特記事項 |
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WB
1/500. Predicted molecular weight: 28 kDa. |
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/3000. |
IP
1/30. |
mIHC
1/4000. |
ターゲット情報
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機能
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. -
組織特異性
Expressed in renal collecting tubules. -
関連疾患
Diabetes insipidus, nephrogenic, autosomal -
配列類似性
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
ドメイン
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
翻訳後修飾
Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. -
細胞内局在
Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated. - Information by UniProt
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参照データベース
- Entrez Gene: 359 Human
- Entrez Gene: 11827 Mouse
- Entrez Gene: 25386 Rat
- Omim: 107777 Human
- SwissProt: P41181 Human
- SwissProt: P56402 Mouse
- SwissProt: P34080 Rat
- Unigene: 130730 Human
see all -
別名
- ADH water channel antibody
- AQP 2 antibody
- AQP CD antibody
see all
画像
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Fluorescence multiplex immunohistochemical analysis of the human kidney (Formalin/PFA-fixed paraffin-embedded sections).
Panel A: merged staining of anti-Hexokinase 1 (ab150423, green; Opal™690), anti-Angiotensin Converting Enzyme 1 (ab254222, gray; Opal™520) and anti-Aquaporin 2 (ab199975, red; Opal™570) on human kidney. Panel B: anti-Aquaporin 2 stained on collecting tubules. Panel C: anti-Angiotensin Converting Enzyme 1 stained on proximal tubules. Panel D: anti-Hexokinase 1 stained on distal tubules and collecting tubules. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
The section was incubated in three rounds of staining: in the order of ab150423 at 1/250 dilution (4.224 μg/ml), ab254222 at 1/4000 dilution (0.141 μg/ml) and ab199975 at 1/4000 dilution (0.152 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of human kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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All lanes : Anti-Aquaporin 2 antibody [EPR21080] (ab199975) at 1/500 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Mouse kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 28 kDaBlocking/dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds.
We recommend customers optimize their lysis buffer and sample preparation for WB. This protein might aggregate during heating of samples.
The molecular mass is consistent with literature (PMID 27405971; PMID 26109089).
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of mouse kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of rat kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse kidney tissue labeling Aquaporin 2 (green) with ab199975 at 1/3000 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive luminal membrane staining and tubular cytoplasmic staining on mouse kidney is observed (PMID: 9688853; 15703994; 19546380). Heat-mediated antigen retrieval was performed using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
Nuclear counterstain: DAPI (blue).
Permeabilization: 0.2% Triton X-100Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary ab150077 at 1/1000 dilution.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed rat kidney tissue labeling Aquaporin 2 (green) with ab199975 at 1/3000 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive luminal membrane staining and tubular cytoplasmic staining on rat kidney is observed (PMID: 9688853; 15703994; 19546380). Heat-mediated antigen retrieval was performed using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
Nuclear counterstain: DAPI (blue).
Permeabilization: 0.2% Triton X-100Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary ab150077 at 1/1000 dilution.
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Aquaporin 2 was immunoprecipitated from 0.35 mg mouse kidney lysate with ab199975 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse kidney lysate 10 μg (input).
Lane 2: ab199975 IP in mouse kidney lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199975 in mouse kidney lysate (-).Blocking/dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds
We recommend customers optimize their lysis buffer and sample preparation for IP. This protein might aggregate during heating of samples.
The molecular mass is consistent with the literature (PMID 27405971; PMID 26109089).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (8)
ab199975 は 8 報の論文で使用されています。
- Kurimoto J et al. Mineralocorticoids induce polyuria by reducing apical aquaporin-2 expression of the kidney in partial vasopressin deficiency. Endocr J 70:295-304 (2023). PubMed: 36450452
- Li M et al. Micropeptide MIAC inhibits the tumor progression by interacting with AQP2 and inhibiting EREG/EGFR signaling in renal cell carcinoma. Mol Cancer 21:181 (2022). PubMed: 36117171
- Wang R et al. Pdcd10-Stk24/25 complex controls kidney water reabsorption by regulating Aqp2 membrane targeting. JCI Insight 6:N/A (2021). PubMed: 34156031
- Qiu Y et al. Upregulation of AQP2 mediated by transcription factor FOXO1 inhibits TGF-ß-induced fibrosis in human urothelial cells. Exp Ther Med 22:1388 (2021). WB ; Human . PubMed: 34650636
- Bernhardt A et al. High salt diet-induced proximal tubular phenotypic changes and sodium-glucose cotransporter-2 expression are coordinated by cold shock Y-box binding protein-1. FASEB J 35:e21912 (2021). PubMed: 34533842
- Unno R et al. Deregulated MTOR (mechanistic target of rapamycin kinase) is responsible for autophagy defects exacerbating kidney stone development. Autophagy 16:709-723 (2020). PubMed: 31257986
- Jia L et al. Differential expression and inhibitory effects of aquaporins on the development of adenomyosis. Mol Med Rep 22:3840-3850 (2020). PubMed: 32901888
- Liu T et al. Zika Virus Infection Induces Acute Kidney Injury Through Activating NLRP3 Inflammasome Via Suppressing Bcl-2. Front Immunol 10:1925 (2019). PubMed: 31474993