Anti-Aquaporin 1 抗体 [1/22] (ab9566)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.co.jp/ps/products/9/ab9566/Images/ab9566-17269-anti-aquaporin-1-antibody-1-22-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)")
Key features and details
- Mouse monoclonal [1/22] to Aquaporin 1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-Aquaporin 1 antibody [1/22]
Aquaporin 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1/22] to Aquaporin 1 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, Flow Cytmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Sheep, Cow
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免疫原
Synthetic peptide:
GQVEEYDLDDDINSRVEMKPK
, corresponding to amino acids 249-269 of Aquaporin 1. -
エピトープ
Intracellular C-terminal AQP 1 epitope (active channel loop) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
1/22 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9566の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
|
| IHC-P | (11) |
Use at an assay dependent concentration.
|
| Flow Cyt |
Use 1-2µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
|
Flow Cyt
Use 1-2µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. -
組織特異性
Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. -
配列類似性
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
ドメイン
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 358 Human
- Entrez Gene: 25240 Rat
- Omim: 107776 Human
- SwissProt: P29972 Human
- SwissProt: P29975 Rat
- Unigene: 76152 Human
- Unigene: 1618 Rat
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別名
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)Immunohistochemical staining of aquaporin 1 in rat brain.
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![Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.co.jp/ps/products/9/ab9566/Images/ab9566-17267-anti-aquaporin-1-antibody-1-22-flow-cytometry.jpg)
Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (ab9566)Overlay histogram showing HEK293 cells stained with ab9566 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9566, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.co.jp/ps/products/9/ab9566/Images/ab9566-279831-anti-aquaporin-1-antibody-1-22-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)Immunohistochemical analysis of exocrine pancreas cells labeling Aquaporin 1 with ab9566.
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![Western blot - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.co.jp/ps/products/9/ab9566/Images/ab9566-17268-anti-aquaporin-1-antibody-1-22-western-blot.jpg)
Western blot - Anti-Aquaporin 1 antibody [1/22] (ab9566)Anti-Aquaporin 1 antibody [1/22] (ab9566) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Additional bands at: 50 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (54)
ab9566 は 54 報の論文で使用されています。
- Moroki T et al. Databases for technical aspects of immunohistochemistry: 2021 update. J Toxicol Pathol 34:161-180 (2021). PubMed: 33976473
- Yin Z et al. RIPK1 is a negative mediator in Aquaporin 1-driven triple-negative breast carcinoma progression and metastasis. NPJ Breast Cancer 7:53 (2021). PubMed: 33980862
- Xie H et al. FoxM1 promotes Wnt/ß-catenin pathway activation and renal fibrosis via transcriptionally regulating multi-Wnts expressions. J Cell Mol Med 25:1958-1971 (2021). PubMed: 33434361
- Makiishi S et al. Carnitine/organic cation transporter 1 precipitates the progression of interstitial fibrosis through oxidative stress in diabetic nephropathy in mice. Sci Rep 11:9093 (2021). PubMed: 33907247
- Huang OS et al. Altered Iris Aquaporin Expression and Aqueous Humor Osmolality in Glaucoma. Invest Ophthalmol Vis Sci 62:34 (2021). PubMed: 33616622