Anti-Aquaporin 1 抗体 [1/22] (ab9566)
Key features and details
- Mouse monoclonal [1/22] to Aquaporin 1
- Suitable for: WB, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-Aquaporin 1 antibody [1/22]
Aquaporin 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1/22] to Aquaporin 1 -
由来種
Mouse -
特異性
This antibody recognises Aquaporin 1 -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Sheep, Cow
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免疫原
Synthetic peptide:
GQVEEYDLDDDINSRVEMKPK
, corresponding to amino acids 249-269 of Aquaporin 1. -
エピトープ
Intracellular C-terminal AQP 1 epitope (active channel loop)
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
1/22 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab9566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). | |
| Flow Cyt | Use 1-2µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. -
組織特異性
Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. -
配列類似性
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
ドメイン
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 358 Human
- Entrez Gene: 25240 Rat
- Omim: 107776 Human
- SwissProt: P29972 Human
- SwissProt: P29975 Rat
- Unigene: 76152 Human
- Unigene: 1618 Rat
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別名
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
see all
画像
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![Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.co.jp/ps/products/9/ab9566/Images/ab9566-17267-anti-aquaporin-1-antibody-1-22-flow-cytometry.jpg)
Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (ab9566)Overlay histogram showing HEK293 cells stained with ab9566 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9566, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions. -
![Western blot - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.co.jp/ps/products/9/ab9566/Images/ab9566-17268-anti-aquaporin-1-antibody-1-22-western-blot.jpg)
Western blot - Anti-Aquaporin 1 antibody [1/22] (ab9566)Anti-Aquaporin 1 antibody [1/22] (ab9566) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Additional bands at: 50 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
プロトコール
参考文献 (39)
ab9566 は 39 報の論文で使用されています。
- Gee MT et al. Expression of SLC4A11 protein in mouse and rat medulla: a candidate transporter involved in outer medullary ammonia recycling. Physiol Rep 7:e14089 (2019). PubMed: 31124301
- Wagoner MD et al. Feeder-free differentiation of cells exhibiting characteristics of corneal endothelium from human induced pluripotent stem cells. Biol Open 7:N/A (2018). PubMed: 29685994
- González-Marrero I et al. Systemic Hypertension Effects on the Ciliary Body and Iris. An Immunofluorescence Study with Aquaporin 1, Aquaporin 4, and Na?, K? ATPase in Hypertensive Rats. Cells 7:N/A (2018). PubMed: 30428541
- Palacio-Mancheno PE et al. Hyperosmolarity induces notochordal cell differentiation with aquaporin3 upregulation and reduced N-cadherin expression. J Orthop Res 36:788-798 (2018). PubMed: 28853179
- Bellezza G et al. Prognostic implication of aquaporin 1 overexpression in resected lung adenocarcinoma. Interact Cardiovasc Thorac Surg 25:856-861 (2017). PubMed: 29106595
