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Anti-APRIL/TNFSF13 抗体 [Sacha-1] (ab16085)

Submit a review Q&A (3)
Flow Cytometry - Anti-APRIL/TNFSF13 antibody [Sacha-1] (ab16085)
  • Immunocytochemistry - Anti-APRIL/TNFSF13 antibody [Sacha-1] (ab16085)

Key features and details

  • Rat monoclonal [Sacha-1] to APRIL/TNFSF13
  • Suitable for: Flow Cyt, ICC
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-APRIL/TNFSF13 antibody [Sacha-1]
    APRIL/TNFSF13 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [Sacha-1] to APRIL/TNFSF13

  • 由来種

    Rat

  • アプリケーション

    適用あり: Flow Cyt, ICCmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Fusion protein corresponding to Human APRIL/TNFSF13 aa 100-250. Fused to amino acids 16 - 108 of human headless, ACRP30.
    Database link: O75888

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Proprietary Purification

  • 特記事項（精製）

    Purified from concentrated hybridoma tissue culture supernatant.

  • ポリ/モノ

    モノクローナル

  • クローン名

    Sacha-1

  • アイソタイプ

    IgG2a

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab16085の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use a concentration of 1 µg/ml.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC
Use a concentration of 5 µg/ml.
特記事項
Flow Cyt
Use a concentration of 1 µg/ml.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC
Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能

    Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.

  • 組織特異性

    Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.

  • 配列類似性

    Belongs to the tumor necrosis factor family.

  • 翻訳後修飾

    The precursor is cleaved by furin.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • A proliferation inducing ligand antibody
      • A proliferation-inducing ligand antibody
      • APRIL antibody
      • CD256 antibody
      • TALL-2 antibody
      • TALL2 antibody
      • TNF related death ligand antibody
      • TNF- and APOL-related leukocyte expressed ligand 2 antibody
      • TNF-related death ligand 1 antibody
      • TNF13_HUMAN antibody
      • TNFSF13 antibody
      • TRDL-1 antibody
      • TRDL1 antibody
      • Tumor necrosis factor (ligand) superfamily, member 13 antibody
      • Tumor necrosis factor ligand superfamily member 13 antibody
      • Tumor necrosis factor like protein ZTNF2 antibody
      • Tumor necrosis factor related death ligand 1 antibody
      • UNQ383/PRO715 antibody
      • ZTNF2 antibody
      see all

    画像

    • Flow Cytometry - Anti-APRIL/TNFSF13 antibody [Sacha-1] (ab16085)
      Flow Cytometry - Anti-APRIL/TNFSF13 antibody [Sacha-1] (ab16085)

      ab16085 at 1ug/ml detecting  membrane-bound human APRIL/TNFSF13 on HEK 293T cells by FACS analysis.

      HEK 293T cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of a non-cleavable mouse A proliferation-inducing ligand (thick line). Cells were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1ug/ml of MAb to APRIL (Sacha-1). After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.

    • Immunocytochemistry - Anti-APRIL/TNFSF13 antibody [Sacha-1] (ab16085)
      Immunocytochemistry - Anti-APRIL/TNFSF13 antibody [Sacha-1] (ab16085)

      Immunostaining of HEK-293T cells transfected with a murine APRIL/TNFSF13 expression plasmid (left panels), or mock transfected (right panel).

      Three days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5ug/ml ab16085 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.

    参考文献 (0)

    ab16085 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab16085 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Question

    Could we buy, the HEK293T lysates used to test this antibody?
    How this antibody was tested for human cross reactivity?

    Read More
    Answer

    Thank you for your email. I am sorry for the delay as lab took more time than anticipated.

    We may be able to provide you the positive control lysates for ab16088. This would be whole cell lysate of HEK 293T cells transfected with an expression plasmid for human APRIL (aa 1-250) fused at the C-terminus to a FLAG®-tag. We used the same lysate to test ab16088. However because ab16085 cross react with mouse and human so this antibody might also cross react.

    Could you let me if you are still interested in buying this lysate? I would be able to provide you the catalogue number soon.

    Once again thank you very much for having patience!

    Read More

    Question

    Could we buy, the HEK293T lysates used to test this antibody?
    How this antibody was tested for human cross reactivity?

    Read More
    Answer

    Thank you for contacting us.

    I can confirm that, I have sent reminder to our collaborator lab. I will send you the requested details as soon as I receive a reply from them.

    Many thanks for having patience!

    Read More

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM no signal. We have tested the antibody on T cells (activated and not activated) as well as macrophages (resting and activated) and have not seen any staining above isotype. SAMPLE Murine macrophages and T cells. Human T cells. PRIMARY ANTIBODY Sacha-1/human,mouse/facs buffer/1:50/30 minutes, washed with perm wash buffer from IC kit DETECTION METHOD FACS and LSR POSITIVE AND NEGATIVE CONTROLS USED Positive controls for were T cells and macrophages (activated and resting) and negative controls were B cells as well as isotype controls. ANTIBODY STORAGE CONDITIONS 4 degrees SAMPLE PREPARATION Mouse splenocytes were ACK treated to purify lymphocytes. Human whole blood was ACK treated. NUMBER OF CELLS USED Roughly 2 X 10 7 cells per sample. PERMEABILIZATION STEP Cells were permeablized using e bioscience kit. And fixed after staining was complete. Antibody was also tested under non permabilizing conditions and without fixation. BLOCKING CONDITIONS 2% mouse serum, diluted in FACS buffer at 4 degrees for fifteen mintues. SECONDARY ANTIBODY PharMingen/rabbit(?)/facs buffer/ 1:50/30 min., wased with perm wash buffer from IC Kit HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? I have tried detecting APRIL staining in a higher expressing population, I have tried looking at both resting and activated cell populations, in human and mouse cells. ADDITIONAL NOTES I have talked to tech support and found out the antibody was tested against transfected cells. I need to know if you have data demonstrating this antibody can recognize phisological levels of APRIL in either human or murine cells.

    Read More
    Answer

    Thank you for your enquiry and patience. Regarding ab16085 (APRIL antibody), this antibody has not been tested on endogeneous APRIL. This would be difficult because APRIL is rapidly cleaved in the cells by furin and secreted. ab16085 was tested in FACS using HEK 293T cells that were transfected with an espression plasmid enabling surface expression of a non-cleavable mouse APRIL. I apologize that this information was missing from our datasheet - we will update this datasheet. Could you please let me know if you have tested this antibody in immunocytochemistry experiments? I am sorry that the product you received did not perform as stated on the datasheet. Since certain information was missing from the ab16085 datasheet, I am going to offer you a free of charge replacement order. Please let me know which Abcam product you would like to receive as a replacement. I apologize for the inconvenience and I look forward to your reply.

    Read More

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