Application
Western blot
Sample
Human Cell lysate - whole cell (1 lane : A549 / 2 lane : H460 human lung cancer c)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Specification
1 lane : A549 / 2 lane : H460 human lung cancer c
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 2% BSA in 1X TBST
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG(H+L)-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
25 second(s)
Bands
Specific: 38 kDa Non-specific: 50, 100 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Yong Sun Lee
Verified customer
投稿 May 22 2018