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This product was changed from ascites to tissue culture supernatant on 2nd February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Our Abpromise guarantee covers the use of ab1906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21163940|
|IP||Use at an assay dependent concentration. PubMed: 21163940|
|WB||1/1000 - 1/10000. Predicted molecular weight: 38 kDa.|
|IHC-P||1/1000 - 1/100000. Antigen retrieval is not essential but may optimise staining.
The staining intensity of formalin-fixed paraffin embedded tissues may be significantly improved by pretreatment methods such as: 70% Formic acid for 10-30 minutes at room temperature or Hydrolytic autoclaving.
|IHC-Fr||1/1000 - 1/100000.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 22896615
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Blocked with 5% milk for 1 hour at 22°C.
Incubated with the primary antibody in 5% milk for 16 hours at 4°C.
ab1906 staining Apolipoprotein E in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/200 in TBS/BSA/azide) for 2 hours at 21°C. An undiluted biotin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
ab1906 staining Apolipoprotein E in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab1906 at 1/100 in blocking solution for 16h at 4°C. An Alexa Fluor® 647 conjugated goat anti-mouset polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Apolipoprotein E expression can be observed in Muller cells.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"